Virus diseases of perennial trees and vines have characteristics not amenable to study using small model annual plants. Unique disease symptoms such as graft incompatibilities and stem pitting cause considerable crop losses. Also, viruses in these long-living plants tend to accumulate complex populations of viruses and strains. Considerable progress has been made in understanding the biology and genetics of Citrus tristeza virus (CTV) and in developing it into a tool for crop protection and improvement. The diseases in tree and vine crops have commonalities for which CTV can be used to develop a baseline. The purpose of this review is to provide a necessary background of systems and reagents developed for CTV that can be used for continued progress in this area and to point out the value of the CTV-citrus system in answering important questions on plant-virus interactions and developing new methods for controlling plant diseases.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1146/annurev-phyto-080614-120012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Complex interplay: The interactions between citrus tristeza virus and its host.
Virology
January 2025
Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA; Department of Plant Pathology, University of Florida, Gainesville, FL, USA. Electronic address:
Citrus tristeza virus (CTV) is one of the largest and most economically important RNA viruses infecting plants. CTV's interactions with various citrus hosts can result in three diseases: quick decline, stem pitting, or seedling yellows. Studying CTV poses several challenges owing to its significant genetic diversity and the highly specific occurrence of disease symptoms when different genotypes infect different citrus hosts.
View Article and Find Full Text PDFApplying infectious clones and untargeted metabolite profiling to characterize citrus tristeza virus-induced stem pitting in citrus.
Sci Rep
November 2024
Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
Article Synopsis
- Citrus tristeza virus (CTV) leads to economically damaging stem pitting in certain citrus varieties, but the mechanisms behind this damage are still not entirely understood.
- Researchers used infectious clones of CTV to investigate how stem pitting occurs in 'Duncan' grapefruit and 'Mexican' lime by replacing specific open reading frames (ORFs) that are thought to influence this condition.
- The study found that certain ORFs, particularly p18 and p23, were associated with increased stem pitting, and the analysis of metabolites revealed significant differences in compounds related to stress response, offering potential insights for improving CTV management techniques.
Intra-Host Citrus Tristeza Virus Populations during Prolonged Infection Initiated by a Well-Defined Sequence Variant in .
Viruses
August 2024
Department of Plant Pathology, University of Florida, Gainesville, FL 32611, USA.
Due to the error-prone nature of viral RNA-dependent RNA polymerases, the replication of RNA viruses results in a diversity of viral genomes harboring point mutations, deletions, insertions, and genome rearrangements. Citrus tristeza virus (CTV), a causal agent of diseases of economically important citrus species, shows intrinsic genetic stability. While the virus appears to have some mechanism that limits the accumulation of single-nucleotide variants, the production of defective viral genomes (DVGs) during virus infection has been reported for certain variants of CTV.
View Article and Find Full Text PDFSerological and Molecular Detection of Citrus Tristeza Virus: A Review.
Microorganisms
July 2024
State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, School of Life Sciences, School of Public Health, Xiamen University, Xiamen 361102, China.
(CTV) is a globally pervasive and economically significant virus that negatively impacts citrus trees, leading to substantial reductions in fruit yield. CTV occurs within the phloem of infected plants, causing a range of disease phenotypes, such as stem pitting (SP), quick decline (QD), and other detrimental diseases. Research on CTV is challenging due to the large size of its RNA genome and the diversity of CTV populations.
View Article and Find Full Text PDFAn Engineered Citrus Tristeza Virus (T36CA)-Based Vector Induces Gene-Specific RNA Silencing and Is Graft Transmissible to Commercial Citrus Varieties.
Phytopathology
November 2024
Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, U.S.A.
A protein-expressing citrus tristeza virus-based vector construct, pT36CA-V1.3, obtained from a California isolate of the T36 strain (T36CA), was retooled into a virus-induced gene silencing system intended for use with studies of California citrus. Virus-induced gene silencing constructs engineered with a truncated () gene sequence in the sense or antisense orientation worked equally well to silence the endogenous gene.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!