AI Article Synopsis
- Recent pseudorabies outbreaks in vaccinated farms in China prompted a study to analyze the genetic characteristics of local PRV strains, specifically HeN1 and JS.
- Comparison with other PRV genomes revealed significant sequence divergence, categorizing Chinese strains as genotype II, distinct from genotype I found in European and American strains.
- The study suggests that long-term independent evolution of these strains may explain the poor effectiveness of the Bartha vaccine in protecting against genotype II PRV infections in pigs.
Article Abstract
Recently pseudorabies outbreaks have occurred in many vaccinated farms in China. To identify genetic characteristics of pseudorabies virus (PRV) strains, we obtained the genomic sequences of PRV strains HeN1 and JS, which were compared to 4 PRV genomes and 729 partial gene sequences. PRV strains isolated in China showed marked sequence divergence compared to European and American strains. Phylogenetic analysis revealed that for the first time PRV can be divided into 2 distinct clusters, with Chinese strains being genotype II and PRVs isolated from other countries being genotype I. Restriction fragment length polymorphism analysis confirmed differences between HeN1 and Bartha strains, as did the presence of unique insertion/deletion polymorphisms and microsatellites. This divergence between the two genotypes may have been generated from long-term, independent evolution, which could also explain the low efficacy of the Bartha vaccine in protecting pigs infected with genotype II PRV.
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| http://dx.doi.org/10.1016/j.virol.2015.04.013 | DOI Listing |
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