AI Article Synopsis

  • The study explored how the triazole-linked acridine ligand GL15 interacts with the tetrahymena telomeric repeat sequence d(TG4T) and polyguanylic acid (poly(G)) using atomic force microscopy (AFM) and voltammetry.
  • GL15 was found to promote G-quadruplex formation in both DNA sequences, showing time-dependent interactions and structural changes detectable through microscopy and electrical measurements.
  • While both Na(+) and K(+) ions facilitated G-quadruplex formation, K(+) specifically helped create well-structured tetra-molecular quadruplexes, leading to two distinct complex structures with d(TG4T) and poly(G).

Article Abstract

The interactions of the Tetrahymena telomeric repeat sequence d(TG4T) and the polyguanylic acid (poly(G)) sequence with the quadruplex-targeting triazole-linked acridine ligand GL15 were investigated using atomic force microscopy (AFM) at a highly oriented pyrolytic graphite and voltammetry at a glassy carbon electrode. GL15 interacted with both sequences, in a time dependent manner, and G-quadruplex formation was detected. AFM showed the adsorption of quadruplexes as small d(TG4T) and poly(G) spherical aggregates and large quadruplex-based poly(G) assemblies, and voltammetry showed the decrease and disappearance of GL15 and guanine oxidation peak currents and appearance of the G-quadruplex oxidation peak. The GL15 molecule strongly stabilized and accelerated G-quadruplex formation in both Na(+) and K(+) ion-containing solution, although only K(+) promoted the formation of perfectly aligned tetra-molecular G-quadruplexes. The small-molecule complex with the d(TG4T) quadruplex is discrete and approximately globular, whereas the G-quadruplex complex with poly(G) is formed at a number of points along the length of the polynucleotide, analogous to beads on a string.

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Source
http://dx.doi.org/10.1021/acs.analchem.5b00743DOI Listing

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