Aims: To compare the apical extrusion of Enterococcus faecalis after instrumentation with three different Ni-Ti rotary instruments- An in vitro study.
Settings And Design: In vitro study Methods and Material: Forty freshly extracted mandibular premolars were mounted in bacteria collection apparatus and root canals were contaminated with a suspension of Enterococcus faecalis. The contaminated teeth were divided into 4 groups of 10 teeth each according to rotary system used for instrumentation: Group1: Hyflex files, Group 2: GTX files, Group 3: Protaper files and Group 4: control group (no instrumentation). Bacteria extruded after preparations were collected into vials and microbiological samples were incubated in BHI broth for 24 hrs. The colony forming units were determined for each sample.
Statistical Analysis Used: Statistical analysis was done using one way ANOVA followed by post hoc independent " t" test.
Results: GTX files extruded least amount of bacteria followed by Hyflex files. Maximum extrusion of E. faecalis was seen in rotary Protaper group.
Conclusion: Least amount of extrusion was seen with GTX files followed by Hyflex files and then rotary Protaper system.
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http://dx.doi.org/10.4103/0970-9290.156813 | DOI Listing |
Unlabelled: Guanosine triphosphate (GTP) is essential for macromolecular biosynthesis, and its intracellular levels are tightly regulated in bacteria. Loss of the alarmone (p)ppGpp disrupts GTP regulation in , causing cell death in the presence of exogenous guanosine and underscoring the critical importance of GTP homeostasis. To investigate the basis of guanosine toxicity, we performed a genetic selection for spontaneous mutations that suppress this effect, uncovering an unexpected link between GTP synthesis and glycolysis.
View Article and Find Full Text PDFBMC Microbiol
January 2025
Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Benha, Egypt.
Background: Novel platforms using nanotechnology-based medicines have exponentially increased in our daily lives. The unique characteristics of metal oxide and noble metals nanoparticles make them suitable for different fields including antimicrobial agents, cosmetics, textiles, wound dressings, and anticancer drug carriers.
Methods: This study focuses on the biosynthesis of small-sized SNPs using exo-metabolites of Fusarium oxysporum via bioprocess optimization using Plackett-Burman (PBD) and central composite designs (CCD) while evaluating their multifaceted bioactivities.
Front Microbiol
December 2024
Shenzhen Centre for Disease Control and Prevention, Shenzhen, China.
Background: The emergence of , which can confer resistance to phenicols and oxazolidinones in spp., poses a growing public health threat.
Methods: 102 -positive enterococci (OPEs) including various species were isolated from feces of 719 healthy volunteers in a Shenzhen community, China.
J Arthroplasty
January 2025
Helios ENDO-Klinik, Hamburg, Germany.
Background: Periprosthetic joint infections (PJI) are one of the most devastating complications of total knee arthroplasty (TKA). Patients who have chronic kidney disease (CKD) are more vulnerable to PJI. We aimed to answer the following questions: 1) What are the commonly observed pathogens in PJI after TKA in CKD patients, and do they differ from those in non-CKD patients? and 2) What are the risk factors for PJI after TKA in CKD patients?
Methods: Patients who underwent surgery due to a chronic PJI of the TKA were retrospectively enrolled.
J Appl Microbiol
January 2025
Department of Bacteriology, Animal Health Research Institute (AHRI), Zagazig Branch, Agriculture Research Center (ARC), 44516, Zagazig, Egypt.
Aims: The present study aimed to detect the frequency of vancomycin resistance and virulence genes` profiles of multi-drug resistant (MDR) enterococcal isolates from different sources and to investigate the sequence heterogeneity between the esp genes of MDR and vancomycin-resistant E. faecalis isolates from chicken and human sources.
Methods And Results: Conventional phenotypic methods identified 91 isolates (60.
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