Objective: We studied the serological response between the special regions on the Torque teno sus virus 2 (TTSuV2) ORF1 coded protein and the porcine sera from conventional pigs.
Methods: Based on a Chinese TTSuV2 strain from Guangdong province, two overlapped virus proteins were expressed from Escherichia coli. Then, purified recombinant TTSuV2 ORF1a and TTSuV2 ORF1ab proteins were used as the antigens in the Western Blotting and ELISA assay.
Results: The recombinant TTSuV2 ORF1a and TTSuV2 ORF1ab proteins were identified with the special tag monoclonal antibody. The results of the ELISA tests shown that there were significant relationships between two groups of dates from the recombinant TTSuV2 ORF1a and TTSuV2 ORF1ab proteins antigenic assay. The results of the following Western Blotting assay indicated that the TTSuV2-specific IgG antibodies were contained in pig sera.
Conclusion: The truncated TTSuV2 ORF1a protein (positions 168 to 346 corresponding to TTSuV2 GDIMA1) contains important B cell epitopes which can stimulate immune system antibody secretion. The truncated TTSuV2 ORF1a protein could be effective in TTSuV2 immunodiagnosis.
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Development of an indirect ELISA assay for the detection of IgG antibodies against the ORF1 of Torque teno sus viruses 1 and 2 in conventional pigs.
Vet Microbiol
October 2015
Centre de Recerca en Sanitat Animal (CReSA)-Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Campus UAB, 08193 Bellaterra, Barcelona, Spain. Electronic address:
Torque teno sus viruses (TTSuV, family Anelloviridae) cause long lasting and persistent infection in pigs under subclinical scenarios, and are potentially linked to several economically important swine diseases. Currently, little is known about swine immune response against TTSuV infections. In this study, an ELISA assay was developed based on the ORF1-A recombinant protein of two known TTSuVs, namely TTSuV1 (genus Iotatorquevirus) and TTSuV2 (genus Kappatorquevirus).
View Article and Find Full Text PDF[Serological tests of the truncated TTSuV2 ORF1 recombinant proteins with the porcine sera].
Wei Sheng Wu Xue Bao
February 2015
Objective: We studied the serological response between the special regions on the Torque teno sus virus 2 (TTSuV2) ORF1 coded protein and the porcine sera from conventional pigs.
Methods: Based on a Chinese TTSuV2 strain from Guangdong province, two overlapped virus proteins were expressed from Escherichia coli. Then, purified recombinant TTSuV2 ORF1a and TTSuV2 ORF1ab proteins were used as the antigens in the Western Blotting and ELISA assay.
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