Extracellular low-molecular-weight DNA in blood of irradiated rats was sequenced for the first time. The screening of sequences in the DDBJ database displayed homology of various parts of the rodent genome. Sequences of low-molecular-weight DNA in rat's plasma are enriched with G/C pairs and long interspersed elements relative to rat genome. DNA sequences in blood of rats irradiated at the doses of 8 and 100 Gy have marked distinctions. Data of sequencing of extracellular DNA from normal humans and with pathology were analyzed. DNA sequences of irradiated rats differ from the human ones by a wealth of long interspersed elements. This new knowledge lays the foundation for development of minimally invasive technologies of diagnosing the probability of pathology and controlling the adaptive resources of people in extreme environments.
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Upregulation of p52-ZER6 (ZNF398) increases reactive oxygen species by suppressing metallothionein-3 in neuronal cells.
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Article Synopsis
- Protein post-translational modification (PTM) is crucial for regulating protein function, and understanding the proportion of modified proteins, known as PTM stoichiometry, is important for studying these modifications.
- The researchers developed a new method called "STO-MS+" that improves upon the original "STO-MS" technique by using a better mass tag and a label-free analysis to enhance measurement efficiency and precision.
- STO-MS+ was successfully applied to measure the stoichiometry of three specific PTMs, showcasing significant advancements in the analysis of post-translational modifications in biological samples.
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