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Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into the Islet-Like Cells: the Role of Extracellular Matrix Proteins. | LitMetric

AI Article Synopsis

  • Recent studies show that pancreatic islet implantation is an effective treatment for type 1 diabetes, but there's a shortage of donor islets.
  • This research investigates how extracellular matrix proteins (collagen, laminin, and vitronectin) affect the transformation of bone marrow stem cells into insulin-producing cells.
  • The findings highlight that laminin significantly promotes the development of insulin-producing cells from bone marrow stem cells, while vitronectin and collagen do not have the same effect.

Article Abstract

Pancreatic islet implantation has been recently shown to be an efficient method of treatment for type 1 diabetes. However, limited availability of donor islets reduces its use. Bone morrow would provide potentially unlimited source of stem cells for generation of insulin-producing cells. This study was performed to evaluate the influence of extracellular matrix proteins like collagen, laminin, and vitronectin on bone marrow mesenchymal stem cells (BM-MSCs) transdifferentiation into islet-like cells (ILCs) in vitro. To our knowledge, this is the first report evaluating the importance of vitronectin in transdifferentiation of BM-MSCs into ILCs. Rat BM-MSCs were induced to ILCs using four-step protocol on plates coated with collagen type IV, laminin type I and vitronectin type I. Quantitative real-time PCR was performed to detect gene expression related to pancreatic β cell development. The induced cells expressed islet-related genes including: neurogenin 3, neurogenic differentiation 1, paired box 4, NK homeobox factor 6.1, glucagon, insulin 1 and insulin 2. Laminin but not collagen type IV or vitronectin enhanced expression of insulin and promoted formation of islet-like structures in monolayer culture. Laminin triggered transdifferentiation of BM-MSCs into ILCs.

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Source
http://dx.doi.org/10.1007/s00005-015-0340-3DOI Listing

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