AI Article Synopsis
- The crystal structures of prostaglandin F synthase (PGF) from two protozoa, Leishmania major and Trypanosoma cruzi, have been resolved with and without the cofactor NADP at varying resolutions (2.6 Å to 1.25 Å).
- These structures were analyzed using molecular replacement techniques and achieved R factors indicating good quality of the data (less than 18.6% for R and less than 22.9% for Rfree).
- PGF is identified as a potential therapeutic target in treating infections caused by these protozoa.
Article Abstract
The crystal structures of prostaglandin F synthase (PGF) from both Leishmania major and Trypanosoma cruzi with and without their cofactor NADP have been determined to resolutions of 2.6 Å for T. cruzi PGF, 1.25 Å for T. cruzi PGF with NADP, 1.6 Å for L. major PGF and 1.8 Å for L. major PGF with NADP. These structures were determined by molecular replacement to a final R factor of less than 18.6% (Rfree of less than 22.9%). PGF in the infectious protozoa L. major and T. cruzi is a potential therapeutic target.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427172 | PMC |
| http://dx.doi.org/10.1107/S2053230X15006883 | DOI Listing |
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