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Overestimation of free light chain antigen excess rate.
Clin Chim Acta
April 2015
Laboratory Medicine, Department of Microbiology and Immunology, University Hospitals Leuven and Experimental Laboratory Immunology, KU Leuven, Herestraat 49, Leuven 3000, Belgium.
Background: Free light chains (FLC) are useful biomarkers for diagnosis and follow-up of plasma cell disorders. FLC quantification is encumbered by non-linearity and antigen excess (>4-fold difference between results obtained at the 2000- and 100-fold dilution).
Methods: FLC concentration was measured with Freelite® reagents on the BNII, using 100- and 2000-fold dilutions in 3645 samples.
Interference testing.
Clin Biochem Rev
August 2008
Department of Chemical Pathology, Pathology Queensland, Princess Alexandra Hospital, Woolloongabba, Qld 4102, Australia.
* Interference occurs when a substance or process falsely alters an assay result. * Interferences are classified as endogenous or exogenous. Endogenous interference originates from substances present in the patient's own specimen.
View Article and Find Full Text PDFInhibition of acetylcholine esterase and choline esterase by benzethonium chloride and avoidance of the benzethonium chloride carry-over inhibitory effect.
Eur J Clin Chem Clin Biochem
August 1997
Department of Clinical Chemistry, University Hospitals Leuven, Belgium.
It has been shown that benzethonium chloride produces linear mixed-type inhibition of choline esterase and acetylcholine esterase. These enzymes also show-reagent-carry-over inhibition if the enzyme activities are measured in plastic cuvettes in which previously protein has been determined by the alkaline benzethonium chloride method. Choline esterase is about 10-fold more sensitive to benzethonium chloride than acetylcholine esterase.
View Article and Find Full Text PDFMulticentre evaluation of the Bayer DAX system.
Eur J Clin Chem Clin Biochem
December 1993
Servizio Chimica e Microscopia Clinica, Stabilimento Ospedaliero di Pordenone.
The analytical performance of the DAX, a high-throughput random access analyser, was studied according to ECCLS guidelines (ECCLS Document Vol. 3, No. 2, Beuth Verlag, Berlin, 1986) in a multicentre evaluation involving four laboratories.
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