AI Article Synopsis
- This study examines the structural variations of a guanine-rich DNA sequence linked to the human c-kit oncogene using techniques like circular dichroism spectroscopy and size exclusion chromatography.
- The original sequence, ckit21, is found to exist as a blend of single and multiple G-quadruplex structures.
- Mutations can change the structure from parallel to antiparallel G-quadruplexes and eliminate multimeric forms, with temperature also affecting these conformations as observed in CD and SEC results.
Article Abstract
The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature size exclusion chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplexes. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392991 | PMC |
| http://dx.doi.org/10.1155/2013/631875 | DOI Listing |
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