Acidovorax sp. CHX100 has a remarkable ability for growth on short cycloalkanes (C5-C8) as a sole source of carbon and energy under aerobic conditions via an uncharacterized mechanism. Transposon mutagenesis of Acidovorax sp. CHX100 revealed a novel cytochrome P450 monooxygenase (CYP450chx) which catalyzed the transformation of cyclohexane to cyclohexanol. Primer walking methods categorized CYP450chx as cytochrome P450 class I taking into account its operon structure: monooxygenase, FAD oxidoreductase, and ferredoxin. CYP450chx was successfully cloned and expressed in Escherichia coli JM109. The activity of CYP450chx was demonstrated by means of the indole co-oxidation. Biotransformation capability of CYP450chx was confirmed through the catalysis of cycloalkanes (C5-C8) to their respective cyclic alcohols.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00253-015-6599-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enlighting Electron Routes In Oxyfunctionalizing Synechocystis sp. PCC 6803.
Chembiochem
January 2024
Department of Microbial Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318, Leipzig, Germany.
Phototrophic microorganisms, like cyanobacteria, are gaining attention as host organisms for biocatalytic processes with light as energy source and water as electron source. Redox enzymes, especially oxygenases, can profit from in-situ supply of co-substrates, i. e.
View Article and Find Full Text PDFLight-driven redox biocatalysis on gram-scale in Synechocystis sp. PCC 6803 via an in vivo cascade.
Plant Biotechnol J
October 2023
Helmholtz-Centre for Environmental Research - UFZ, Permoserstr, Leipzig, Germany.
The photosynthetic light reaction in cyanobacteria constitutes a highly attractive tool for productive biocatalysis, as it can provide redox reactions with high-energy reduction equivalents using sunlight and water as sources of energy and electrons, respectively. Here, we describe the first artificial light-driven redox cascade in Synechocystis sp. PCC 6803 to convert cyclohexanone to the polymer building block 6-hydroxyhexanoic acid (6-HA).
View Article and Find Full Text PDFRational orthologous pathway and biochemical process engineering for adipic acid production using Pseudomonas taiwanensis VLB120.
Metab Eng
March 2022
Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Permoserstraße 15, 04318, Leipzig, Germany.
Microbial bioprocessing based on orthologous pathways constitutes a promising approach to replace traditional greenhouse gas- and energy-intensive production processes, e.g., for adipic acid (AA).
View Article and Find Full Text PDFWhole-cell biocatalysis using the Acidovorax sp. CHX100 Δ6HX for the production of ω-hydroxycarboxylic acids from cycloalkanes.
N Biotechnol
January 2021
Helmholtz-Centre for Environmental Research - UFZ GmbH, Department of Solar Materials, Permoserstr. 15, 04318 Leipzig, Germany. Electronic address:
Omega hydroxycarboxylic acids (ω-HAs) possess two functional groups, a hydroxyl group and a carboxyl group, and are essential precursors for the production of biodegradable polyester polymers. In this work, an Acidovorax mutant was investigated as a whole-cell biocatalyst for the conversion of cycloalkanes to their respective ω-hydroxycarboxylic acids. This Acidovorax sp.
View Article and Find Full Text PDFBiocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in Pseudomonas taiwanensis VLB120.
Biotechnol Bioeng
February 2018
Department of Solar Materials, Helmholtz-Centre for Environmental Research-UFZ GmbH, Leipzig, Germany.
Chemical synthesis of lactones from cycloalkanes is a multi-step process challenged by limitations in reaction efficiency (conversion and yield), atom economy (by-products) and environmental performance. A heterologous pathway comprising novel enzymes with compatible kinetics was designed in Pseudomonas taiwanensis VLB120 enabling in-vivo cascade for synthesizing lactones from cycloalkanes. The respective pathway included cytochrome P450 monooxygenase (CHX), cyclohexanol dehydrogenase (CDH), and cyclohexanone monooxygenase (CHXON) from Acidovorax sp.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!