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Unexpectedly fast transfer of positron-emittable artificial substrate into N-terminus of peptide/protein mediated by wild-type L/F-tRNA-protein transferase. | LitMetric

Unexpectedly fast transfer of positron-emittable artificial substrate into N-terminus of peptide/protein mediated by wild-type L/F-tRNA-protein transferase.

Amino Acids

Division of Chemistry and Biochemistry, Department of Medical and Bioengineering Science, Chemical Biology Area, Okayama University, 1-1-1, Tsushimanaka, Okayama, 700-0082, Japan,

Published: June 2015

This article demonstrates the fastest enzymatic introduction of a positron emission tomography (PET) probe into acceptor peptides/proteins. It is site-specifically introduced at the basic N-terminus of the acceptors by using L/F-transferase in combination with aminoacyl-tRNA synthetase, namely the NEXT-A/PET reaction. Estimated from kinetic analysis, the transfer efficiency of O-(2-fluoromethyl)-L-tyrosine as an artificial amino acid PET probe mediated by the wild-type transferase is almost as good as that of the natural substrate, phenylalanine.

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Source
http://dx.doi.org/10.1007/s00726-015-1989-yDOI Listing

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