Background: Aminopeptidase N (APN; EC 3.4.11.2) is a membrane dimeric metallopeptidase involved in differentiation, development, and proliferative processes of several tissues. Recent studies have demonstrated the increased expression and activity of this enzyme in several cancers. However, there are no available data about the impact of this peptidase in the biological aggressiveness and the survival of colorectal cancer (CRC) patients.
Methods: The activity and mRNA expression of APN in tumor tissue (n = 81) and plasma (n = 40) of patients with CRC of low and high grades and stages were prospectively analyzed by fluorimetric and quantitative reverse transcriptase-polymerase chain reaction methods. Data obtained in adenoma and CRC were compared with those from the surrounding normal mucosa. Classic clinical and pathological parameters were stratified following APN data and analyzed for 5-year survival.
Results: mRNA levels of APN (ANPEP) were lower in colorectal adenomas and adenocarcinomas than in the surrounding uninvolved mucosa (Kruskal-Wallis, P < 0.001). Aminopeptidase N activity in CRC tissue was higher in patients with better overall survival (log-rank P < 0.05, Cox analysis P < 0.05). By contrast, higher plasmatic APN activity correlated with worse overall survival (log-rank P < 0.01, Cox analysis P < 0.05).
Conclusions: Aminopeptidase N activity in tissue and plasma from CRC patients is an independent prognostic factor of 5-year survival. The determination of APN activity levels in the plasma may be a safe, minimally invasive, and inexpensive way to define the aggressiveness of CRC in daily practice.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/JIM.0000000000000199 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of a β-glucan-enriched diet on biomarkers of oxidative stress, energy metabolism and lysosomal function in muscle tissue of European grayling ( L.).
J Vet Res
December 2024
Institute of Biology, Pomeranian University in Słupsk, 76-200 Słupsk, Poland.
Introduction: The grayling ( L.) has several advantages over other fish species that make it attractive for aquaculture and invest it with importance for food security. The study assessed the effects of a β-glucan-enriched diet on biomarkers of oxidative stress, energy metabolism and lysosomal function in muscle tissue of European grayling ( L.
View Article and Find Full Text PDFContinuous Cropping of Patchouli Alleviate Soil Properties, Enzyme Activities, and Bacterial Community Structures.
Plants (Basel)
December 2024
School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, China.
(Patchouli), an essential medicinal plant in the family, faces significant challenges under continuous cropping (CC) obstacles. This study examined the rhizospheric soil bacterial communities of patchouli under four different CC years, zero (CK), one (T1), two (T2), and three (T3) years through high-throughput 16S rRNA gene amplicon sequencing. Results showed long-term CC led to significant soil properties and enzyme activity shifts.
View Article and Find Full Text PDFSexual and Metabolic Differences in Hippocampal Evolution: Alzheimer's Disease Implications.
Life (Basel)
November 2024
Experimental and Clinical Physiopathology Research Group CTS-1039, Department of Health Sciences, Faculty of Health Sciences, University of Jaen, Las Lagunillas University Campus, 23009 Jaen, Spain.
Sex differences in brain metabolism and their relationship to neurodegenerative diseases like Alzheimer's are an important emerging topic in neuroscience. Intrinsic anatomic and metabolic differences related to male and female physiology have been described, underscoring the importance of considering biological sex in studying brain metabolism and associated pathologies. The hippocampus is a key structure exhibiting sex differences in volume and connectivity.
View Article and Find Full Text PDFMetAP2 as a Therapeutic Target for Obesity and Type 2 Diabetes: Structural Insights, Mechanistic Roles, and Inhibitor Development.
Biomolecules
December 2024
Department of Biology Education, Daegu University, 201, Daegudae-ro, Gyeongsan-si 38453, Gyeongsangbuk-do, Republic of Korea.
Type 2 Diabetes Mellitus (T2DM) and obesity are globally prevalent metabolic disorders characterized by insulin resistance, impaired glucose metabolism, and excessive adiposity. Methionine aminopeptidase 2 (MetAP2), an intracellular metalloprotease, has emerged as a promising therapeutic target due to its critical role in regulating lipid metabolism, energy balance, and protein synthesis. This review provides a comprehensive analysis of MetAP2, including its structural characteristics, catalytic mechanism, and functional roles in the pathophysiology of T2DM and obesity.
View Article and Find Full Text PDFThe Expression of Proteases and the Oligopeptide Transporter in the Yolk Sac Membrane, Proventriculus, and Small Intestine During the Development of Embryo.
Biology (Basel)
November 2024
Department of Biology and Biotechnology, Faculty of Science, Hashemite University, P.O. Box 330127, Zarqa 13133, Jordan.
The role of the yolk sac membrane (YSM) and digestive tract in the processing of egg yolk proteins during embryogenesis is unexplored in the duck . Here, we investigated in the duck embryo the function of the YSM, proventriculus, and small intestine in protein digestion and uptake. We tested the expression of aminopeptidase N () and the oligopeptide transporter as well as the expression of cathepsin B () and cathepsin D () lysosomal genes in the YSM during incubation days 12, 14, 16-18, 20, 22, 24, 26, and 28 (the day of hatch).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!