AI Article Synopsis
- Brown adipose tissue (BAT) helps prevent obesity by generating energy through a process called uncoupled respiration, and researchers identified around 1,500 long non-coding RNAs (lncRNAs) specific to BAT using RNA sequencing.
- Among these lncRNAs, lnc-BATE1 plays a crucial role in maintaining the identity and thermogenic function of BAT; inhibiting it disrupts the balance between activation of brown fat and repression of white fat genes.
- The study highlights the importance of lnc-BATE1 in brown adipogenesis by showing its interaction with a protein called heterogeneous nuclear ribonucleoprotein U, providing valuable insights into fat-specific lncRNAs and their roles in adipose tissue biology.
Article Abstract
Brown adipose tissue (BAT) protects against obesity by promoting energy expenditure via uncoupled respiration. To uncover BAT-specific long non-coding RNAs (lncRNAs), we used RNA-seq to reconstruct de novo transcriptomes of mouse brown, inguinal white, and epididymal white fat and identified ∼1,500 lncRNAs, including 127 BAT-restricted loci induced during differentiation and often targeted by key regulators PPARγ, C/EBPα, and C/EBPβ. One of them, lnc-BATE1, is required for establishment and maintenance of BAT identity and thermogenic capacity. lnc-BATE1 inhibition impairs concurrent activation of brown fat and repression of white fat genes and is partially rescued by exogenous lnc-BATE1 with mutated siRNA-targeting sites, demonstrating a function in trans. We show that lnc-BATE1 binds heterogeneous nuclear ribonucleoprotein U and that both are required for brown adipogenesis. Our work provides an annotated catalog for the study of fat depot-selective lncRNAs and establishes lnc-BATE1 as a regulator of BAT development and physiology.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4429916 | PMC |
| http://dx.doi.org/10.1016/j.cmet.2015.04.003 | DOI Listing |
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