The callus growth kinetics allows identifying the appropriate moment for callus pealing and monitoring the accumulation of primary and secondary metabolites. The physic nut (Jatropha curcas L.) is a plant species used for biofuel production due to its high oil content; however, this plant presents a great amount of bioactive compounds which can be useful for industry. The aim of this research was to establish a calli growth curve and to evaluate the fatty acid profile of crude oil extracted from callus. The callus growth kinetics presented a sigmoid standard curve with six distinct phases: lag, exponential, linear, deceleration, stationary, and decline. Total soluble sugars were higher at the inoculation day. Reducing sugars were higher at the inoculation day and at the 80th day. The highest percentage of ethereal extract (oil content) was obtained at the 120th day of culture, reaching 18 % of crude oil from the callus. The calli produced medium-chain and long-chain fatty acids (from 10 to 18 carbon atoms). The palmitic acid was the fatty acid with the highest proportion in oil (55.4 %). The lipid profile obtained in callus oil was different from the seed oil profile.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12010-015-1618-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Optimizing In Vitro Propagation of Schönland Using Leaf, Root, and Inflorescence.
Plants (Basel)
January 2025
Instituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain.
, a species native to South Africa, is characterized by its limited growth and scarcity, contributing to high production costs. Countries like China and Turkey are known for exporting globally. Tissue culture offers an efficient method for mass-producing unique and beautiful species such as This study tested Murashige and Skoog (MS) basal media supplemented with various concentrations of IBA (0.
View Article and Find Full Text PDFThe cell colony development is connected with the accumulation of embryogenesis-related proteins and dynamic distribution of cell wall components in in vitro cultures of Fagopyrum tataricum and Fagopyrum esculentum.
BMC Plant Biol
January 2025
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, St. Jagiellonska 28, Katowice, 40-032, Poland.
Background: Due to the totipotency of plant cells, which allows them to reprogram from a differentiated to a dedifferentiated state, plants exhibit a remarkable regenerative capacity, including under in vitro culture conditions. When exposed to plant hormones, primarily auxins and cytokinins, explant cells cultured in vitro can undergo differentiation through callus formation. Protoplast culture serves as a valuable research model for studying these processes in detail.
View Article and Find Full Text PDFExosome-Like Vesicles from Callus Enhanced Wound Healing by Reducing LPS-Induced Inflammation.
J Microbiol Biotechnol
November 2024
Preclinical Research Center, Daegu Gyeongbuk Medical Innovation Foundation (K-MEDI hub), Daegu 41061, Republic of Korea.
(LE), a medicinal plant from the Boraginaceae family, is traditionally used in East Asia for its therapeutic effects on skin conditions, including infections, inflammation, and wounds. Recently, the role of extracellular vesicles (EVs) as mediators of intercellular communication that regulate inflammation and promote tissue regeneration has garnered increasing attention in the field of regenerative medicine. This study investigates exosome-like vesicles derived from LE callus (LELVs) and their potential in enhancing wound healing.
View Article and Find Full Text PDFOptimizing extrusion-based 3D bioprinting of plant cells with enhanced resolution and cell viability.
Biofabrication
January 2025
Mechanical Engineering, Tsinghua University, A421 Lee Shau Kee Building, Tsinghua Uniersity, Haidian District, Beijing, 100084, CHINA.
3D bioprinting of plant cells has emerged as a promising technology for plant cell immobilization and related applications. Despite the numerous progress in mammal cell printing, the bioprinting of plant cells is still in its infancy and needs further investigation. Here, we present a systematic study on optimizing the 3D bioprinting of plant cells, using carrots as an example, towards enhanced resolution and cell viability.
View Article and Find Full Text PDFEfficient regeneration protocol for producing true-to-type oil palm ( (jacq.) through somatic embryogenesis from immature male inflorescence.
Heliyon
January 2025
ICAR-Indian Institute of Oil Palm Research, Pedavegi, 534 435, Eluru (Dt), Andhra Pradesh, India.
Producing homogeneous planting material in oil palm poses a significant challenge, which can be addressed through somatic embryogenesis. This study successfully achieved somatic embryogenesis using immature male inflorescence from Tenera hybrid. Modified Eeuwens medium yielded better results than the Murashige and Skoog (MS) and CHU (N6) media when supplemented with 2,4-D, picloram and α-naphthaleneacetic acid (NAA).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!