To separate and identify chemical signals which induce Thesium chinense haustorium formation, the components of T. chinense roots secretion collected with XAD-4 resin were detected by GC-MS. The effect of DMBQ as exogenous signals to induce haustorium formation in T. chinense was studied. Fifty-three compounds of 9 types had been detected, including hydrocarbons, esters, organic acids, ketones, alcohols, nitrogen containing compounds, phenolic acids, aldehyde and quinine. It is worth noting that the 2, 5-di-tert-butyl-1,4-benzoquinone has the core structure of 1,4-benzoquinone, which may play an important role in the parasitic relationship of Prunella vulgaris and T. chinense: DMBQ worked effectively on inducing haustoria, but induction effects vary widely in different concentrations. DMBQ with the concentration of 1 μmol x L(-1) showed the best effect of the inducing ability with a ratio of 110.52 when treated to induce haustoria.
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Langmuir
January 2025
School of Chemistry and Chemical Engineering, Jiangsu Province Hi-Tech Key Laboratory for Biomedical Research, Southeast University, Nanjing, Jiangsu 211189, P. R. China.
This study aims to improve the signal-to-noise ratio (SNR) of chemiluminescence immunoassay (CLIA) by increasing the amount of protein immobilized on the surface of the magnetic bead (MB). Proteins are macromolecules with three-dimensional structures, and merely increasing the density of functional groups on the two-dimensional surface of the MB cannot significantly enhance protein immobilization. Therefore, we grafted spatially extended functional polymer to not only increase the density of functional groups on the MB surface but also expand their distribution in three-dimensional space, ultimately increasing protein immobilization.
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January 2025
State Key Laboratory of Electromechanical Integrated Manufacturing of High-performance Electronic Equipment, School of Mechano-Electronic Engineering, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, 710071, China.
In this research, a novel detection method employing rare-earth upconversion nanoparticle (UCNP) as the core, coated with MnO nanosheets is designed, which formed a color and fluorescence dual-responsive UCNP composite material, MnO-modified NaYF:Yb,Tm@NaYF. By enabling both colorimetric and fluorescence methods simultaneously, this composite material allows for the detection of glucose concentration under different conditions, while exhibiting strong resistance to environmental interference, chemical stability, and accuracy. To further enhance the sensitivity of the detection method, a photonic crystals (PCs)-PDMS array where polymethyl methacrylate PCs are deposited onto a substrate composed of PDMS-glass slice with hydrophobic surfaces is developed.
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January 2025
Faculty of Health Sciences, University of Macau, Taipa, Macau.
Ion channels play a crucial role in cardiac functions, and their activities exhibit dynamic changes during heart development. However, the precise function of ion channels in human heart development remains elusive. In this study, we utilized human embryonic stem cells (hESCs) as a model to mimic the process of human embryonic heart development.
View Article and Find Full Text PDFInt J Med Sci
January 2025
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Nephrotoxicity remains a significant concern associated with tyrosine kinase inhibitors, such as dasatinib (DASA). Previous studies have shown that DASA can induce renal tubular cell death, contributing to its nephrotoxic effects. In contrast, naringenin (NGN) is known for its antioxidant and anti-inflammatory properties.
View Article and Find Full Text PDFInt J Med Sci
January 2025
Health Management Institute, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing 100853, China.
Receptor-interacting protein 3 (Ripk3) plays a crucial part in acute lung injury (ALI) by regulating inflammation-induced endothelial damage in the lung tissue. The precise mechanisms through which Ripk3 contributes to the endothelial injury in ALI still remain uncertain. In the current research, we employed Ripk3-deficient (Ripk3) mice to examine the role of Ripk3 in ALI progression, focusing on its effects on endothelial cells (ECs), mitochondrial damage and necroptosis.
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