Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system.

J Biosci Bioeng

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan; Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan. Electronic address:

Published: October 2015

Recombinant peroxidase from Arthromyces ramosus, fused with domains of antibody-binding proteins, was successfully obtained by a silkworm larvae expression system. The catalytic activity of the fusion peroxidase was increased 6-fold with the injection of 5-aminolevulinic acid into silkworm larvae as a heme precursor.

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http://dx.doi.org/10.1016/j.jbiosc.2015.02.013DOI Listing

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