AI Article Synopsis
- The study introduces a microfluidic device designed for co-culturing cells while varying oxygen levels, allowing for real-time analysis of the protein VEGF165 and its role in cell communication.
- The findings reveal that at low oxygen concentrations (5% O2), CaSki cells migrate faster than HUVEC cells, but this changes at higher oxygen levels (15% O2).
- Results indicate that shorter distances between cells enhance migration speed and implicate VEGF165 and HIF-1α genes in understanding cervical cancer diagnosis and treatment.
Article Abstract
In this work, we report an integrated microfluidic device for cell co-culture under different concentrations of oxygen, in which the secreted protein VEGF165 was on-line qualitatively and semi-quantitatively analyzed by functional nucleic acid, hemin, ABTS and peroxide system. This microfluidic platform allowed investigation of various oxygen and distances effect on cell-to-cell communication. Besides, the microfluidic device was used for real-time analysis of VEGF165 protein by aptamer-functionalized microchannels. Under 5% O2 condition, we found that the migration of CaSki cells was faster than the migration of human umbilical vein endothelial cells. However, the migration of CaSki cells was slower than the migration of HUVECs under 15% O2 condition. Moreover, the shorter intercellular distances, the quicker cells migration. Furthermore, HIF-1α and VEGF165 genes, ROS were analyzed, and the results would provide new perspectives for the diagnosis and medical treatment of cervical cancer.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5386116 | PMC |
| http://dx.doi.org/10.1038/srep09643 | DOI Listing |
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