AI Article Synopsis
- A reversed-phase high-performance liquid chromatography (HPLC) method is developed to measure tilidine in both bulk and its dosage forms.
- The method utilizes an RP-8 column with a methanol-ammonium carbonate solution as the mobile phase and can separate the compounds in about 6.17 minutes.
- It shows a linear response for tilidine concentration up to 3.6 mmol/l, with impressive recovery rates between 98.2% and 101.2%, indicating its efficiency and reliability.
Article Abstract
A reversed-phase high performance liquid chromatographic method for the determination of tilidine in bulk drug and its liquid and solid dosage forms is described. The method uses reversed-phase column RP-8 with methanol -0.2% water solution of ammonium carbonate (75:25,v/v) as the mobile phase and UV detector. The time taken for separation is 6.17 min. The response is linear up to 3.6 mmol/l, with recovery levels ranging from 98.2 to 101.2%. The present method is rapid, simple and reproducible.
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