AI Article Synopsis
- Volatile terpenoids like monoterpenes and sesquiterpenes, which are produced by terpene synthases (TPSs), play important roles in how plants respond to various stresses.
- A full-length cDNA of the RlemTPS4 gene, linked to responses against microbial attack in rough lemon, was isolated and shown to localize in the cytosol of the plant cells.
- The RlemTPS4 enzyme can produce sesquiterpenes, including δ-elemene, and is specifically expressed in the special epithelial cells around oil secretory cavities in the leaves of rough lemon plants.
Article Abstract
Volatile terpenoids such as monoterpenes and sesquiterpenes play multiple roles in plant responses and are synthesized by terpene synthases (TPSs). We have previously isolated a partial TPS gene, RlemTPS4, that responds to microbial attack in rough lemon. In this study, we isolated a full length RlemTPS4 cDNA from rough lemon. RlemTPS4 localized in the cytosol. The recombinant RlemTPS4 protein was obtained using a prokaryotic expression system and GC-MS analysis of the terpenes produced by the RlemTPS4 enzymatic reaction determined that RlemTPS4 produces some sesquiterpenes such as δ-elemene. The RlemTPS4 gene was specifically expressed in specialized epithelial cells surrounding the oil secretory cavities in rough lemon leaf tissue.
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| http://dx.doi.org/10.1016/j.jplph.2015.03.013 | DOI Listing |
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