Polymorphonuclear neutrophils (PMNs) are essential cellular constituents in the innate host response, and their recruitment to the lungs and subsequent ubiquitous phagocytosis controls primary respiratory infection. Cystic fibrosis pulmonary disease is characterized by progressive pulmonary decline governed by a persistent, exaggerated inflammatory response dominated by PMNs. The principal contributor is chronic Pseudomonas aeruginosa biofilm infection, which attracts and activates PMNs and thereby is responsible for the continuing inflammation. Strategies to prevent initial airway colonization with P. aeruginosa by augmenting the phagocytic competence of PMNs may postpone the deteriorating chronic biofilm infection. Anti-P. aeruginosa IgY antibodies significantly increase the PMN-mediated respiratory burst and subsequent bacterial killing of P. aeruginosa in vitro. The mode of action is attributed to IgY-facilitated formation of immobilized bacteria in aggregates, as visualized by fluorescence microscopy and the induction of increased bacterial hydrophobicity. Thus, the present study demonstrates that avian egg yolk immunoglobulins (IgY) targeting P. aeruginosa modify bacterial fitness, which enhances bacterial killing by PMN-mediated phagocytosis and thereby may facilitate a rapid bacterial clearance in airways of people with cystic fibrosis.
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http://dx.doi.org/10.1128/IAI.02970-14 | DOI Listing |
Sci Rep
January 2024
Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran-Qom Express Way, Tehran, 3319118651, Iran.
Pseudomonas aeruginosa (PA) is a multidrug-resistant (MDR) opportunistic pathogen causing severe hospital-, and community-acquired infections worldwide. Thus, the development of effective immunotherapy-based treatments is essential to combat the MDR-PA infections. In the current study, we evaluated the protective efficacy of polyclonal avian antibodies raised against inactivated whole cells of the PAO1 strain in murine models of acute pneumonia and burn wound.
View Article and Find Full Text PDFInnate Immun
October 2022
Anesthesiology and Intensive Care, 174469Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.
Ventilator associated pneumonia (VAP) caused by is a cause of morbidity and mortality in critically ill patients. The spread of pathogens with anti-microbial resistance mandates the investigation of novel therapies. Specific polyclonal anti- IgY-antibodies (IgY) might be effective for VAP caused by The objective of this study was to investigate if intravenous IgY decreases the lower airway concentration of in VAP.
View Article and Find Full Text PDFFront Microbiol
September 2022
Department of Clinical Microbiology, Rigshospitalet, Copenhagen University Hospital, København, Denmark.
Introduction: Urinary tract infections (UTIs) with are a severe problem in disposed patients in modern healthcare. establishes recalcitrant biofilm infections and can develop antibiotic resistance. Gargling with avian egg yolk anti- antibodies (IgY) has shown clinical effect in preventing onset of chronic lung infections in patients with cystic fibrosis (CF).
View Article and Find Full Text PDFMol Immunol
August 2022
Departamento de Ciências Patológicas - Universidade Estadual de Londrina, Brazil. Electronic address:
Multi-drug resistant Pseudomonas aeruginosa is a gram-negative bacillus responsible for nosocomial infections. Immunoglobulin Y (IgY) is a chicken immunoglobulin used for research, immunodiagnosis, and immunotherapy. IgY presents antimicrobial properties and it is under investigation for use as an adjunct to prophylactic therapies.
View Article and Find Full Text PDFMol Immunol
January 2022
Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Electronic address:
Background: Pseudomonas aeruginosa sepsis is associated with unacceptably high mortality and, for many of those who survive, long-term morbidity. The aims of this study were to production of IgY against chimeric protein pilQ-pilA-DSL region and killed- whole cell Pseudomonas aeruginosa O1 (PAO1) strain and their efficacy for immunoprophylaxis of sepsis caused by P. aeruginosa in a rabbit model.
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