AI Article Synopsis
- The study cloned a gene called OsOPT7 from rice, which is regulated by iron deficiency and is found in root tips and shoots.
- It was shown that OsOPT7 does not transport certain forms of iron and failed to help yeast mutants grow when added, suggesting its role may not be directly in iron transport.
- Knockout plants (opt7-1) had increased expression of iron-responsive genes even when iron was sufficient, indicating that OsOPT7 might play a role in providing iron for essential functions in rice.
Article Abstract
The molecular mechanism of iron (Fe) uptake and transport in plants are well-characterized; however, many components of Fe homeostasis remain unclear. We cloned iron-deficiency-regulated oligopeptide transporter 7 (OsOPT7) from rice. OsOPT7 localized to the plasma membrane and did not transport Fe(III)-DMA or Fe(II)-NA and GSH in Xenopus laevis oocytes. Furthermore OsOPT7 did not complement the growth of yeast fet3fet4 mutant. OsOPT7 was specifically upregulated in response to Fe-deficiency. Promoter GUS analysis revealed that OsOPT7 expresses in root tips, root vascular tissue and shoots as well as during seed development. Microarray analysis of OsOPT7 knockout 1 (opt7-1) revealed the upregulation of Fe-deficiency-responsive genes in plants grown under Fe-sufficient conditions, despite the high Fe and ferritin concentrations in shoot tissue indicating that Fe may not be available for physiological functions. Plants overexpressing OsOPT7 do not exhibit any phenotype and do not accumulate more Fe compared to wild type plants. These results indicate that OsOPT7 may be involved in Fe transport in rice.
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| http://dx.doi.org/10.1007/s11103-015-0315-0 | DOI Listing |
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