AI Article Synopsis
- Deep sequencing has significantly advanced the research on rapidly mutating RNA viruses, especially the hepatitis C virus, by allowing detailed analysis of viral diversity and evolution.
- The study utilized genotype-independent high-throughput pyrosequencing to reconstruct near full-length hepatitis C virus genomes from clinical samples across various subtypes.
- Results indicated that the reconstruction success varied widely (from 79.95% to 99.64%), but there was no significant correlation between the extent of genome reconstruction and factors like viral load or the number of HCV reads.
Article Abstract
Background: Deep sequencing has a deep impact on the study of rapidly mutating RNA viruses, such as hepatitis C virus, proving to be an invaluable tool for analyzing virus diversity and evolution.
Aim: Genotype-independent high-throughput pyrosequencing was used to obtain near full length hepatitis C virus genome sequence reconstruction directly from clinical samples.
Methods: Samples from hepatitis C virus infected subjects harbouring different subtypes (1a, 1b, 2c) were analyzed (viral load range: 1.2-20.8 × 10(6)IU/ml). Data were generated with a modified sequence-independent single primer amplification method followed by 454 sequencing.
Results: the extent of reconstructed hepatitis C virus genome varied from 79.95% to 99.64%. No correlation between extent of genome reconstruction and either viral load (r=0.4857, p=0.3556) or number of HCV reads (r=0.08571, p=0.9194) was observed.
Conclusion: This study describes a protocol for obtaining whole genome sequences from different hepatitis C virus patients with different genotypes in a single sequencing run.
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| http://dx.doi.org/10.1016/j.dld.2015.03.015 | DOI Listing |
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