AI Article Synopsis
- Lipopeptides (LP) are important antibiotic compounds produced by bacteria, and their biosynthesis is largely controlled by complex enzymes called nonribosomal peptide synthetases (NRPS).
- The study discovered that the proteins ClpA and ClpP play a crucial role in regulating the production of LP massetolide in Pseudomonas fluorescens SS101, affecting various gene expressions linked to cell function.
- By analyzing changes in gene and protein expression in mutant strains, the research revealed new connections in the regulatory networks controlling LP biosynthesis, highlighting the significance of ClpAP and associated proteins like MassAR, DnaK, and DnaJ.
Article Abstract
Background: Lipopeptides (LP) are structurally diverse compounds with potent surfactant and broad-spectrum antibiotic activities. In Pseudomonas and other bacterial genera, LP biosynthesis is governed by large multimodular nonribosomal peptide synthetases (NRPS). To date, relatively little is known about the regulatory genetic network of LP biosynthesis.
Results: This study provides evidence that the chaperone ClpA, together with the serine protease ClpP, regulates the biosynthesis of the LP massetolide in Pseudomonas fluorescens SS101. Whole-genome transcriptome analyses of clpA and clpP mutants showed their involvement in the transcription of the NRPS genes massABC and the transcriptional regulator massAR. In addition, transcription of genes associated with cell wall and membrane biogenesis, energy production and conversion, amino acid transport and metabolism, and pilus assembly were altered by mutations in clpA and clpP. Proteome analysis allowed the identification of additional cellular changes associated to clpA and clpP mutations. The expression of proteins of the citrate cycle and the heat shock proteins DnaK and DnaJ were particularly affected. Combined with previous findings, these results suggest that the ClpAP complex regulates massetolide biosynthesis via the pathway-specific, LuxR-type regulator MassAR, the heat shock proteins DnaK and DnaJ, and proteins of the TCA cycle.
Conclusions: Combining transcriptome and proteome analyses provided new insights into the regulation of LP biosynthesis in P. fluorescens and led to the identification of specific missing links in the regulatory pathways.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332742 | PMC |
| http://dx.doi.org/10.1186/s12866-015-0367-y | DOI Listing |
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