Objective: We studied the ability of Lactobacillus plantarum ZJ8 to bind fumonisins FB1 and FB2.
Methods: The percentage of FB1 and FB2 bound by the strain was measured by HPLC after bacterial cells and FB1 and FB2 were co-incubated in MRS media at 37% for 4 h.
Results: The percentage of FB1- and FB2-binding was 89.9% and 95.0%, respectively. The FBs-binding rate of strain ZJ8 reached the maximum at pH 4. Binding of FBs was poor under alkaline conditions and high temperatures. After acid and SDS treatment, the FBs-binding rate significantly increased. The percentage of FB1- and FB2-binding was 96.8% and 100% for the cell walls of strain ZJ8, respectively. Moreover, about 96.8% FB, and 100% FB, was bound to the peptidoglycan of strain ZJ8' s cell walls.
Conclusions: L. plantarum ZJ8 has potential to remove fumonisins. The peptidoglycan of cell walls from strain ZJ8 was proved to be the main site of FBs-binding.
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Wei Sheng Wu Xue Bao
December 2014
Objective: We studied the ability of Lactobacillus plantarum ZJ8 to bind fumonisins FB1 and FB2.
Methods: The percentage of FB1 and FB2 bound by the strain was measured by HPLC after bacterial cells and FB1 and FB2 were co-incubated in MRS media at 37% for 4 h.
Results: The percentage of FB1- and FB2-binding was 89.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
January 1998
Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China.
The coding region of BmvPK-1 gene of Bombyx mori NPV (Strain ZJ8) is 828 nt long and encodes a 276 aa polypeptide with predicted molecular mass of 32 kD. Dot blot analysis showed its mRNA to be gene is first detectable at 18 h p.i.
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