Nanoparticles (NP) are significant to multiple industrial processes, consumer products and medical applications today. The health effects of many different types of NP, however, are largely unknown. The purpose of this study was to test the effects of 50-nm gold NP coated with poly-N-vinylpyrrolidone (PVP) on mouse macrophage and spleen cells with and without lipopolysaccharide (LPS), testing the hypothesis that the NP would modulate immune responses without being overtly toxic. Gold NP had no effect on macrophage viability and, in the absence of LPS, they had no effect on tumor necrosis factor (TNF)-α production as measured by ELISA. The presence of LPS significantly increased the release of TNFα from the macrophages above no-treatment controls, but increasing gold NP concentration led to decreasing release of TNFα. The reactive oxygen species (ROS) produced by exposed macrophages were also reduced compared to untreated controls, both with and without LPS, suggesting some kind of oxygen radical scavenging. In splenocyte cultures, gold NP had no effect alone, but significantly reduced the release of interleukin (IL)-17 and TNFα triggered by LPS. These results suggest that the gold NP used here are not cytotoxic to immune cells at these concentrations, but may affect cellular responses to infection or inflammation by altering the balance of cytokines.
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http://dx.doi.org/10.3109/1547691X.2015.1035819 | DOI Listing |
J Vasc Anom (Phila)
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Laboratory for Biomaterials and Drug Delivery, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts.
Objective: The current treatment of venous malformations (VMs) consists of medications with systemic toxicity and procedural interventions with high technical difficulty and risk of hemorrhage. Using nanoparticles (NPs) to enhance drug delivery to VMs could enhance efficacy and decrease systemic toxicity. NPs can accumulate in tissues with abnormal vasculature, a concept known as the enhanced permeation and retention (EPR) effect.
View Article and Find Full Text PDFNutr Res
July 2024
Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, University of Florida College of Medicine, Jacksonville, FL 32206.
The role of vitamin D in regulating calcium metabolism and skeletal growth and disease is widely recognized. Indeed, current recommendations for serum vitamin D concentrations are based on these parameters. A serum vitamin D <20 ng/mL is considered deficient, concentrations between 20 and 30 ng/mL are insufficient, and >30 ng/mL is adequate.
View Article and Find Full Text PDFAdv Mater
December 2024
Department of Chemical Sciences and Centre for Advanced Functional Materials, Indian Institute of Science Education and Research (IISER) Kolkata, Mohanpur, 741246, India.
Advancements in stimuli-driven nanoactuators necessitate the discovery of photo-switchable, self-contained semiconductor nanostructures capable of precise mechanical responses. The reversible assembly of 0D CsBiI halide perovskite nanoplatelets (NPLs) between stacked and scattered configurations are demonstrated under light and dark, respectively. This sunlight-triggered perpetual flipping of the NPLs, occurring in less than a minute, is associated with a color change between brown and red.
View Article and Find Full Text PDFPlant Physiol Biochem
December 2024
Department of Pomology, Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt; Biology Research & Studies Institute, Assiut University, Assiut, 71526, Egypt. Electronic address:
Smart nanohybrid technology with potential advantages to plants has recently been developed formanaging the widespread pollution of heavy metals. Herein, we disclose a novel strategy to combat Pb stress in strawberry (Fragaria spp. cv.
View Article and Find Full Text PDFAnal Chem
December 2024
Department of Chemistry, The University of Kansas, Lawrence, Kansas 66045, United States.
We are developing a unique protein identification method that consists of generating peptides proteolytically from a single protein molecule (i.e., peptide fingerprints) with peptide detection and identification carried out using nanoscale electrochromatography and label-free resistive pulse sensing (RPS).
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