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Antibody-free workflows for protein quantification by LC-MS/MS. | LitMetric

Antibody-free workflows for protein quantification by LC-MS/MS.

Bioanalysis

Analytical Biochemistry, Department of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.

Published: January 2016

AI Article Synopsis

Article Abstract

Antibody-free approaches for quantitative LC-MS/MS-based protein bioanalysis are reviewed and critically evaluated, and compared with the more widely used immunoaffinity-based approaches. Antibody-free workflows will be divided into four groups and discussed in the following order: direct analysis of signature peptides after proteolytic digestion; enrichment of target proteins and signature peptides by fractionated protein precipitation; enrichment of target proteins and signature peptides by reversed-phase and ion-exchange solid-phase extraction; and enrichment of target proteins and signature peptides by (antibody-free) affinity-solid-phase extraction.

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Source
http://dx.doi.org/10.4155/bio.14.308DOI Listing

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