The carmine spider mite, Tetranychus cinnabarinus, which is also considered as the red form of Tetranychus urticae, is one of the most serious mite pests on crops. It is capable of rapidly developing resistance to acaricides, and has caused difficulty in controlling. However, the resistance mechanism of this mite remains unclear at molecular level. As a member of main detoxification enzymes, cytochrome P450 monooxygenases (CYPs or P450s) play important roles in the development of acaricide resistance in arthropods. In this study, two novel P450 genes (CYP389B1 and CYP392A26) were identified and characterized from T. cinnabarinus. The opening reading frames (ORFs) of CYP389B1 and CYP392A26 contained 1545 and 1488 nucleotides, which encode 514 and 495 amino acids, respectively. Phylogenetic analysis showed that CYP389B1 and CYP392A26 were most closely related to CYP389B1 and CYP392A4 from T. urticae, respectively. When treated with piperonyl butoxide (PBO), an inhibitor of P450s, the resistance ratio of fenpropathrin-resistant (FeR) strain decreased from 101- to 75-fold, which suggested a correlation between P450 and fenpropathrin-resistance in T. cinnabarinus. Furthermore, constitutive over-expressions of CYP389B1 and CYP392A26 were detected in FeR strain. Meanwhile, the expressions of CYP389B1 and CYP392A26 were inducible in FeR strain after treatment in 6, 12 and 24 h with LC30 of fenpropathrin; especially, the expression of CYP392A26 increased to a markedly high level (20.88-fold higher than in the control) after treatment in 6 h. However, there was no significant difference between treatment and control in susceptible strain. Furthermore, stage specific expression profiles of these two genes did not show significant difference among developing stages, except for eggs, in which the mRNA levels were quite low. The results indicate that CYP389B1 and CYP392A26 were involved in the fenpropathrin-resistance in T. cinnabarinus, and the expression of CYP392A26 was more sensitive to fenpropathrin stress. These findings provide clues for further elucidating the function and regulation mechanism of these two cytochrome P450 genes in T. cinnabarinus.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/j.pestbp.2015.02.009 | DOI Listing |
Insect Mol Biol
October 2016
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Cytochrome P450 monooxygenases (P450s), as an important family of detoxification enzymes, participate in the metabolism of agrochemicals in almost all agricultural pests and play important roles in the development of insecticide resistance. Two P450 genes (CYP389B1 and CYP392A26) were identified and their expression patterns were investigated in our previous study. In this study, four more P450 gene sequences (CYP391A1, CYP384A1, CYP392D11 and CYP392A28) from the Clan 2, Clan 3 and Clan 4 families were identified and characterized.
View Article and Find Full Text PDFPestic Biochem Physiol
March 2015
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China. Electronic address:
The carmine spider mite, Tetranychus cinnabarinus, which is also considered as the red form of Tetranychus urticae, is one of the most serious mite pests on crops. It is capable of rapidly developing resistance to acaricides, and has caused difficulty in controlling. However, the resistance mechanism of this mite remains unclear at molecular level.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!