AI Article Synopsis
- Two-photon microscopy offers enhanced spatial resolution and deeper sample penetration compared to traditional microscopy methods.
- The study introduces a phosphorescent probe made from human serum albumin and an Au-Ag complex, which shows strong luminescence in the infrared range (700-800 nm), peaking at 740 nm.
- This probe is effective for bioimaging, successfully visualizing glioma C6 cells and various tissues using two-photon confocal microscopy, with the ability to adjust the excitation wavelength for better signal contrast.
Article Abstract
Two-photon microscopy reveals several advantages over conventional one since it provides higher spatial resolution as well as deeper penetration into the sample under study. The development of suitable two-photon probes is one of the most challenging tasks in this area. Here we present phosphorescent non-covalent adduct of human serum albumin and Au-Ag alkynyl-diphosphine complex, [Au14Ag4(C2Ph)12(PPh2C6H4PPh2)6][PF6]4, which exhibits high cross section of two-photon-induced luminescence (δTPE) within large near-infrared excitation wavelength region (700-800 nm) with maximum δTPE about 38 GM at 740 nm. This feature makes it a promising probe for multiphoton bioimaging as demonstrated by successful visualization of glioma C6 cells and various tissues by two-photon confocal microscopy both in planar and z-stacking modes. Additionally, the broad excitation region enables optimization of the signal-to-background auto-fluorescence ratio via variation of excitation wavelength.
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| http://dx.doi.org/10.1016/j.jinorgbio.2015.03.014 | DOI Listing |
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