Purpose: To evaluate, in vivo, the effect of fluoride dentifrice and diet control on the demineralization/remineralization processes at the margin of bracket/enamel interface bonded with four different bonding materials, using optical microscopy and fluorescent laser (DIAGNOdent).

Methods: Premolars in 35 subjects (11 to 20 years old) were divided into Group 1 (n = 15) and Group 2 (n = 20). Four bracket/enamel interfaces (near points) and four points at a distance of 2 mm (distant points) were observed. Evaluations were made at the following times: 15 days before bracket bonding (T0), on day of bonding (T1), 1 week after bonding (T2), and 4 weeks after bonding (T3). Subjects received fluoride dentifrice, and toothbrush and were instructed to brush 3x/day, after main meals. Group 1 received the instructions at T1 and Group 2 at T0 and these were reinforced weekly. The Microarch brackets were bonded with four materials: Transbond XT; Concise Ortodôntico; Fuji Ortho LC and Monolok2. DIAGNOdent laser readouts were used for comparison.

Results: Data were statistically analyzed by Mann-Whitney, Wilcoxon and Friedman tests (P < 0.05). Higher readouts were found at nearer points than at distant points; Group 2 presented lower readouts than Group 1; No differences were observed among bonding materials.

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