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A laser-plasma-produced soft X-ray laser at 89 eV generates DNA double-strand breaks in human cancer cells. | LitMetric

A laser-plasma-produced soft X-ray laser at 89 eV generates DNA double-strand breaks in human cancer cells.

J Radiat Res

Laser Electron Accelerator Group, Quantum Beam Science Directorate, Kansai Photon Science Institute, Japan Atonic Energy Agency, 8-1-7 Umemidai, Kizugawa, Kyoto, 619-0215, Japan.

Published: July 2015

AI Article Synopsis

Article Abstract

While it has been expected that X-ray laser will be widely applied to biomedical studies, this has not been achieved to date and its biological effects such as DNA damage have not been evaluated. As a first step for its biological application, we developed a culture cell irradiation system, particularly designed for a plasma-driven soft X-ray laser pulse, to investigate whether the soft X-ray laser is able to induce DNA double strand breaks (DSBs) in living cells or not. The human adenocarcimona cell line A549 was irradiated with the soft X-ray laser at a photon energy of 89 eV and the repair focus formation of the DSBs was assessed by immunofluorescence staining with antiphosphorylated DNA-PKcs (p-DNA-PKcs), ATM (p-ATM) and γ-H2AX antibody. The p-DNA-PKcs, ATM, and γ-H2AX foci were clearly identified after soft X-ray laser irradiation. Furthermore, the increase in the X-ray laser shot number, even from a single shot, results in the increase in p-DNA-PKcs foci. These results are the first evidence that the 89 eV soft X-ray laser is able to induce DSB in living cells. Our study demonstrated that this irradiation system is a useful tool for investigating the radiobiological effect of soft X-ray laser.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497388PMC
http://dx.doi.org/10.1093/jrr/rrv015DOI Listing

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