AI Article Synopsis
- Pulsed field gel electrophoresis (PFGE) is a high-resolution technique that uses alternating electric fields to separate large DNA fragments.
- The method involves digesting bacterial DNA with rare-cutting enzymes and applying an alternating electrical current, allowing DNA fragments to migrate at different speeds based on their size.
- PFGE is widely recognized as the "gold standard" for various applications, including genotyping, genetic fingerprinting, epidemiological studies, and studying DNA damage and repair, and this chapter specifically details its use for differentiating dairy propionibacteria.
Article Abstract
Pulsed field gel electrophoresis (PFGE) is a technique using alternating electric fields to migrate high molecular weight DNA fragments with a high resolution. This method consists of the digestion of bacterial chromosomal DNA with rare-cutting restriction enzymes and in applying an alternating electrical current between spatially distinct pairs of electrodes. DNA molecules migrate at different speeds according to the size of the fragments. Among other things, this technique is considered as the "gold standard" for genotyping, genetic fingerprinting, epidemiological studies, genome size estimation, and studying radiation-induced DNA damage and repair. This chapter describes a PFGE method that can be used to differentiate dairy propionibacteria.
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| http://dx.doi.org/10.1007/978-1-4939-2599-5_21 | DOI Listing |
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