Background And Objectives: For our understanding of the pathogenesis of rheumatoid arthritis (RA), it is important to elucidate the mechanisms underlying early stages of synovitis. Here, synovial cytokine production was investigated in patients with very early arthritis.
Methods: Synovial biopsies were obtained from patients with at least one clinically swollen joint within 12 weeks of symptom onset. At an 18-month follow-up visit, patients who went on to develop RA, or whose arthritis spontaneously resolved, were identified. Biopsies were also obtained from patients with RA with longer symptom duration (>12 weeks) and individuals with no clinically apparent inflammation. Synovial mRNA expression of 117 cytokines was quantified using PCR techniques and analysed using standard and novel methods of data analysis. Synovial tissue sections were stained for CXCL4, CXCL7, CD41, CD68 and von Willebrand factor.
Results: A machine learning approach identified expression of mRNA for CXCL4 and CXCL7 as potentially important in the classification of early RA versus resolving arthritis. mRNA levels for these chemokines were significantly elevated in patients with early RA compared with uninflamed controls. Significantly increased CXCL4 and CXCL7 protein expression was observed in patients with early RA compared with those with resolving arthritis or longer established disease. CXCL4 and CXCL7 co-localised with blood vessels, platelets and CD68(+) macrophages. Extravascular CXCL7 expression was significantly higher in patients with very early RA compared with longer duration RA or resolving arthritis
Conclusions: Taken together, these observations suggest a transient increase in synovial CXCL4 and CXCL7 levels in early RA.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819606 | PMC |
| http://dx.doi.org/10.1136/annrheumdis-2014-206921 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of tocilizumab on endothelial and platelet-derived CXC-chemokines and their association with inflammation and myocardial injury in STEMI patients undergoing primary PCI.
Int J Cardiol
January 2025
Clinic of Cardiology, St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.
Article Synopsis
- Researchers studied the effects of a drug called tocilizumab on heart injury in patients who had serious heart attacks.
- They found that tocilizumab didn't really change the levels of certain chemicals (CXC chemokines) that are linked to heart damage.
- However, they discovered that a different treatment called heparin affected the levels of these chemicals, showing how different medications can impact heart health differently.
Research progress of the chemokine/chemokine receptor axes in the oncobiology of multiple myeloma (MM).
Cell Commun Signal
March 2024
Department of Hematology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Article Synopsis
- The incidence of multiple myeloma (MM) is increasing, and while new treatments have improved outcomes, the disease remains incurable; understanding the chemokine network's role in MM could lead to better biomarkers and therapies.
- This research reviews the complex roles of various chemokines in MM, identifying which can be used as biomarkers and exploring potential treatments that target these chemokines.
- Findings suggest that some chemokines help cancer cells evade treatment while others can enhance immune responses; new therapeutic strategies, including anti-tumor chemokines and combinations with CAR-T therapy, show promise for future clinical use.
Antagonistic Roles of Human Platelet Integrin αIIbβ3 and Chemokines in Regulating Neutrophil Activation and Fate on Arterial Thrombi Under Flow.
Arterioscler Thromb Vasc Biol
September 2023
Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, the Netherlands (C.S., S.L.N.B., J.M.E.M.C., R.R.K., S.P.W., M.J.E.K., J.W.M.H., M.N.).
Background: Platelets and neutrophils are the first blood cells accumulating at sites of arterial thrombus formation, and both cell types contribute to the pathology of thrombotic events. We aimed to identify key interaction mechanisms between these cells using microfluidic approaches.
Methods: Whole-blood perfusion was performed over a collagen surface at arterial shear rate.
Platelet-Derived Biomarkers: Potential Role in Early Pediatric Serious Bacterial Infection and Sepsis Diagnostics.
J Clin Med
October 2022
Department of Pediatrics, Faculty of Medicine, Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania.
Fever is the most common complaint of children who are attending a pediatric emergency department (PED). Most of the fever cases are of viral origin; however, the most common markers, such as leucocyte, neutrophil count, or C-reactive protein, are not sensitive or specific enough to distinguish the etiology of fever, especially if children present at the early phase of infection. Currently, platelets have been attributed a role as important sentinels in viral and bacterial infection pathogenesis.
View Article and Find Full Text PDFSpontaneous abortion is associated with differentially expressed angiogenic chemokines in placenta and decidua.
Arch Gynecol Obstet
September 2023
Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, Alexandroupolis, Greece.
Purpose: Miscarriage is one of the most common complications of pregnancy. Although chromosomal abnormalities of the embryo is a well-known cause of miscarriage, a lot of cases remain unexplained, with immunologic and vascular growth alterations being considered as probable causes. Chemokines are produced by a variety of cells and exhibit several functions including both pro and anti-angiogenic properties.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!