Metallothioneins (MTs) are of low molecular mass, cysteine-rich proteins. They play an important role in the detoxification of heavy metals and homeostasis of intracellular metal ions, and protecting against intracellular oxidative damages. In this study a full-length cDNA of type 2 plant metallothioneins, HbMT2a, was isolated from 25 mM Polyethyleneglycol (PEG) stressed leaves of Hevea brasiliensis by RACE. The HbMT2a was 372bp in length and had a 237bp open reading frame (ORF) encoding for a protein of 78 amino acid residues with molecular mass of 7.772 kDa. The expression of HbMT2a in the detached leaves of rubber tree clone RY7-33-97 was up-regulated by Me-JA, ABA, PEG, H2O2, Cu(2+) and Zn(2+), but down-regulated by water. The role of HbMT2a protein in protecting against metal toxicity was demonstrated in vitro. PET-28a-HbMT2-beared Escherichia coli. Differential expression of HbMT2a upon treatment with 10 °C was observed in the detached leaves of rubber tree clone 93-114 which is cold-resistant and Reken501 which is cold-sensitive. The expression patterns of HbMT2a in the two rubber tree clones may be ascribed to a change in the level of endogenous H2O2.
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http://dx.doi.org/10.1016/j.bbrc.2015.03.175 | DOI Listing |
J Fungi (Basel)
December 2024
National Key Laboratory for Tropical Crop Breeding, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572024, China.
To obtain an effective bacterial biocontrol strain against the fungal pathogen , causing rubber tree red root rot disease, healthy rubber tree tissue from Baisha County, Hainan Province, was selected as the isolation source, and bacterial strains with strong antifungal effects against . were screened. The strain was identified by molecular biology, in vitro root segment tests, pot growth promotion tests, and genome detection.
View Article and Find Full Text PDFMicrobiol Spectr
December 2024
Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
Unlabelled: Plant-associated microbial communities strongly relate to host health and productivity. Still, our knowledge of microbial community spatiotemporal patterns in soil-plant continuum is largely limited. Here, we explored the spatiotemporal dynamics of fungal communities across multiple compartments (phyllosphere, leaf endosphere, soil, rhizosphere, rhizoplane, and root endosphere) of rubber tree in two contrasting seasons collected from Hainan Island and Xishuangbanna.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Post-Graduation Program in Materials Sciences (PPGCM), Federal University of São Carlos (UFSCar), 18052-780, Brazil; Laboratory of Biomaterials, Faculty of Medical Sciences and Health (FCMS), Pontifical Catholic University of São Paulo (PUC-SP), 18030-070, Brazil; Mechanical Engineering Faculty (FEM), State University of Campinas (UNICAMP), 13083-970, Brazil; Post-Graduation Program of Biomaterials and Regenerative Medicine (PPGBMR), Surgery Department, (PUC-SP), 18030-070, Brazil.
Wound healing is a complex process involving a sequence of factors that can be disrupted, negatively impacting the quality of life for patients and overburdening healthcare systems. Advanced dressings obtained by electrospinning are highlighted by the optimization of this process, allowing air exchange and protection against microorganisms. Aiming to develop bioactive dressings, this study investigated the physicochemical, mechanical, microbiological, and in vitro biological properties of membranes containing 25 %, 50 %, 75 %, and 90 % copaiba oil (CO) co-electrospun with poly(L-co-D,L-lactic acid) (PLDLA) and natural rubber latex (NR).
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
December 2024
Engineering Department, Federal University of Lavras (UFLA), C.P. 3037, Lavras, MG, 37200-900, Brazil.
The incorporation of residues in cement matrices is a viable alternative for obtaining new products for civil construction and a sustainable solution for the disposal of materials discarded by industries. In this context, the objective of the study was to evaluate the potential use of quartzite and particles of Hevea brasiliensis in the production of cement-wood panels. Quartzite residues and Hevea brasiliensis wood particles were obtained and characterized.
View Article and Find Full Text PDFMycology
January 2024
Department of Science and Bioinnovation, Faculty of Liberal Arts and Science, Kasetsart University, Nakhon Pathom, Thailand.
Spent mushroom substrate (SMS), a lignocellulosic waste after mushroom production is generally discarded without proper management. There is increasing interest in the sustainable transformation of lignocellulosic waste into high-value products. Within this context, the present study investigated the potential of the SMS from the cultivation of and on rubber tree wood sawdust as substrates for xylooligosaccharides (XOS) production.
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