[Establishment and preliminary analysis of two-dimensional electrophoresis patterns of protein in ginseng callus].

Objective: To establish the two-dimensional electrophoresis (2-DE)maps of callus induced from ginseng root,and to provide scientific method and data support for the study of secondary metabolites biosynthesis pathways of ginseng.

Methods: Total protein was extracted from ginseng callus by three different methods. The best protein extraction method of ginseng callus tissue was determined by the protein yield, purity and SDS-PAGE protein bands. By using 2-DE technology, the 2-DE maps of ginseng callus was established. The mass spectrometry and function on part of the protein were analyzed using MALDI-TOF-TOF and Swiss-Prot software.

Results: Modified phenol extraction method (the third method)was the best protein extraction method of ginseng callus tissue, and it could obtain a high resolution 2-DE map. The second method was lesser. The protein quality of the first method could not be used for the analysis of 2-DE. 12 protein spots of the second and third methods were successfully identified by mass spectrometry.

Conclusion: This study established extraction methods suitable for ginseng callus protein and 2-DE patterns.