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Experimental infection of mice with bovine viral diarrhea virus. | LitMetric

Experimental infection of mice with bovine viral diarrhea virus.

Arch Virol

Department of Animal Biotechnology, College of Ecology and Environmental Science, Kyungpook National University, Sangju, 742-711, Republic of Korea.

Published: June 2015

AI Article Synopsis

  • * Although infected mice showed no clinical symptoms, significant tissue changes were observed, and BVDV was detected in blood and various organs via specific tests (RT-PCR and IHC).
  • * The findings indicate that the spleen is the most reliable tissue for detecting BVDV in mice, marking this research as the first documentation of BVDV infection in this animal model.

Article Abstract

The objective of this study was to test the ability of bovine viral diarrhea virus (BVDV) to infect mice. Two mice each were either mock infected or inoculated with one of three BVDV strains by the intraperitoneal (IP) (n = 8) or intranasal (IN) (n = 8) route. All mice were euthanized at day 7 postinfection (p.i.). None of the infected mice exhibited any clinical signs of illness; however, the tissues harvested after BVDV challenge showed significant histopathological changes. Blood samples from five mice that were injected IP and one mouse that was inoculated IN were positive for BVDV by reverse transcription polymerase chain reaction (RT-PCR). Immunohistochemistry (IHC) was used to assess the presence of viral antigen in the organs of mice infected with three BVDV strains. In IP-injected mice, BVDV antigen was detected in the spleen (5/6), mesenteric lymph nodes (4/6), lymphatic tissue of the lung (3/6), lung (1/6), and stomach (1/6) of the infected mice; however, it was not detected in the liver (0/6) or kidney (0/6). In IN-inoculated mice, BVDV antigen was detected in the lung and mesenteric lymph nodes of one BVDV-infected mouse but was not detected in other tissues. The results of this study suggest that the spleen is the most reliable tissue for BVDV antigen detection using IHC in the IP-injected group. Our study demonstrates that mice can be infected by BVDV. This is the first report of BVDV infection in mice.

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Source
http://dx.doi.org/10.1007/s00705-015-2412-4DOI Listing

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