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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124729 | PLOS |
Lupus Sci Med
January 2025
Dermatology, The University of Texas Southwestern Medical Center, Dallas, Texas, USA
Objective: Metabolic reprogramming plays a critical role in modulating the innate and adaptive immune response, but its role in cutaneous autoimmune diseases, such as cutaneous lupus erythematosus (CLE), is less well studied. An improved understanding of the metabolic pathways dysregulated in CLE may lead to novel treatment options, biomarkers and insights into disease pathogenesis. The objective was to compare metabolomic profiles in the skin and sera of CLE and control patients using liquid chromatography-mass spectrometry (LC-MS).
View Article and Find Full Text PDFPathogens
December 2024
Department of Inorganic and Analytical Chemistry, Faculty of Chemistry, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland.
and are challenging to differentiate using methods such as phenotyping, 16S rRNA sequencing, or protein profiling through matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) due to their close relatedness. This study explores the potential for identifying and by incorporating reference spectra of metabolite profiles, obtained via surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) employing gold nanoparticles (AuNPs), into the Bruker Biotyper database. Metabolite extracts from and cells were prepared using liquid-liquid extraction in a chloroform-methanol-water system.
View Article and Find Full Text PDFSensors (Basel)
January 2025
Engineering Design, KTH Royal Institute of Technology, SE-100 44 Stockholm, Sweden.
Topography estimation is essential for autonomous off-road navigation. Common methods rely on point cloud data from, e.g.
View Article and Find Full Text PDFSensors (Basel)
January 2025
School of Environment and Spatial Informatics, China University of Mining and Technology, Xuzhou 221000, China.
In recent years, mobile laser measurement systems have markedly enhanced the capabilities of deformation detection and defect identification within metro tunnels, attributed to their superior efficiency, precision, and versatility. Nevertheless, challenges persist, including substantial equipment costs, inadequate after-sales support, technological barriers, and limitations in customization. This paper develops a mobile laser measurement system that has been specifically developed for the purpose of detecting deformation in metro tunnels.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
National Institute for Bioprocessing Research and Training, Foster Avenue, Mount Merrion, Blackrock, A94 X099 Dublin, Ireland.
Recombinant Adeno-associated virus (rAAV) is a popular vector for treating genetic diseases caused by absent or defective genes. rAAVs can be produced that contain a therapeutic transgene, i.e.
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