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Introduction: Celiac Disease (CD)-related antibody positivity in children with Type 1 Diabetes (T1D) may fluctuate and become negative spontaneously. There are uncertainties about the optimal tTG-IgA titre and timing of endoscopy in the diagnosis of CD, and this study aimed to contribute to the debate on the tTGA-IgA threshold titre for endoscopy decisions in children with T1D.
Methods: The data of 991 children with T1D who had undergone serologic evaluation for CD were analysed retrospectively.
Diagnosis of West Nile virus infections: Evaluation of different laboratory methods.
World J Virol
December 2024
OIE Reference Center for West Nile Disease, Istituto Zooprofilattico Sperimentale, G. Caporale, Teramo 64100, Italy.
Background: The diagnosis of West Nile virus (WNV) is challenging due to short-term and low-level viremia, flavivirus cross-reactivity, and long immunoglobulin M (IgM) persistence.
Aim: To evaluate different methods for WNV detection [reverse transcription-polymerase chain reaction (RT-PCR), IgM/IgG antibodies, IgG avidity] in serum, cerebrospinal fluid (CSF), and urine samples of patients with confirmed WNV infection.
Methods: The study included patients with confirmed WNV neuroinvasive infection ( = 62), asymptomatic WNV seropositive individuals ( = 22), and individuals with false-positive WNV IgM antibodies ( = 30).
False Positive Babesia microti Result in New-Onset Systemic Lupus Erythematosus Manifesting With Febrile Illness.
Cureus
November 2024
Pathology, Reading Hospital, Tower Health, West Reading, USA.
False positive serologic results are common in systemic lupus erythematosus (SLE) due to the presence of autoantibodies. We present a case of a young patient initially suspected of having a tick-borne disease with a false positive Babesia microti antibody result, and later diagnosed with SLE. Acute babesiosis was excluded after additional laboratory tests such as Babesia polymerase chain reaction (PCR) and blood smear for parasites.
View Article and Find Full Text PDFAntibodies to Anaplasma phagocytophilum in Patients with Human Granulocytic Anaplasmosis Confirmed by Both Polymerase Chain Reaction and Culture.
Am J Med
December 2024
Clinical Laboratories, Westchester Medical Center, Valhalla NY; Department of Pathology, Microbiology and Immunology, New York Medical College, Valhalla, NY.
Background/aims: Sera from patients from a single medical institution in New York State with human granulocytic anaplasmosis established by a positive polymerase chain reaction test (PCR) for Anaplasma phagocytophilum were used to assess the performance of serologic testing. All cases were also confirmed by culture in order to eliminate any false positive PCR samples.
Methods: A nested PCR was performed targeting the heat shock operon of A.
Evaluating the Pathogenic Potential of IgE Targeting Cross-Reactive Carbohydrate Determinants in Dogs.
Animals (Basel)
November 2024
Center for Clinical Veterinary Medicine, Ludwig-Maximilians University Munich, 80539 Munich, Germany.
Cross-reactive carbohydrate determinants (CCDs) are complex N-glycans shared among allergens of plant, insect venom, and nematode origin. In allergic humans, IgE anti-CCD often develop and cause discrepancies between serological and skin tests. Overall, CCD-IgE are believed to be of low pathogenic relevance.
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