The Myb locus encodes the c-Myb transcription factor involved in controlling a broad variety of cellular processes. Recently, it has been shown that c-Myb may play a specific role in hard tissue formation; however, all of these results were gathered from an analysis of intramembranous ossification. To investigate a possible role of c-Myb in endochondral ossification, we carried out our study on the long bones of mouse limbs during embryonic development. Firstly, the c-myb expression pattern was analyzed by in situ hybridization during endochondral ossification of long bones. c-myb positive areas were found in proliferating as well as hypertrophic zones of the growth plate. At early embryonic stages, localized expression was also observed in the perichondrium and interdigital areas. The c-Myb protein was found in proliferating chondrocytes and in the perichondrium of the forelimb bones (E14.5-E17.5). Furthermore, protein was detected in pre-hypertrophic as well as hypertrophic chondrocytes. Gain-of-function and loss-of-function approaches were used to test the effect of altered c-myb expression on chondrogenesis in micromass cultures established from forelimb buds of mouse embryos. A loss-of-function approach using c-myb specific siRNA decreased nodule formation, as well as downregulated the level of Sox9 expression, a major marker of chondrogenesis. Transient c-myb overexpression markedly increased the formation of cartilage nodules and the production of extracellular matrix as detected by intense staining with Alcian blue. Moreover, the expression of early chondrogenic genes such as Sox9, Col2a1 and activity of a Col2-LUC reporter were increased in the cells overexpressing c-myb while late chondrogenic markers such as Col10a1 and Mmp13 were not significantly changed or were downregulated. Taken together, the results of this study demonstrate that the c-Myb transcription factor is involved in the regulation and promotion of endochondral bone formation.
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http://dx.doi.org/10.1016/j.bone.2015.02.031 | DOI Listing |
Food Res Int
February 2025
College of Tea Science, Guizhou University, Guiyang 550025, China. Electronic address:
Volatile terpenoids are major substances responsible for the floral and fruity scents of teas. However, little is known about the regulatory mechanisms of terpenoid biosynthesis pathways in tea plants. 'Zhenfeng Yesheng tea' (ZFYS), a distinctive tea tree germplasm resource in Guizhou province, is known for its unique flavor characterized by a mellow taste and a floral aroma.
View Article and Find Full Text PDFGenes Genomics
January 2025
Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan, 32439, Republic of Korea.
Background: Soil salinity has been a serious threat to agricultural production worldwide, including soybeans. Glycine soja, the wild ancestor of cultivated soybeans, harbors high genetic diversity and possesses attractive rare alleles.
Objective: We conducted a transcriptome analysis of G.
Sci Rep
January 2025
Plant Biotechnology Lab, Department of Botany, Faculty of Science, Dayalbagh Educational Institute (Deemed to be University), Dayalbagh, Agra, 282005, India.
Piper longum, commonly known as long pepper, is highly valued for its bioactive alkaloid piperine, which has diverse pharmaceutical and culinary applications. In this study, we used high-throughput sequencing and de novo transcriptome assembly to analyze the transcriptomes of P. longum leaves, roots, and spikes.
View Article and Find Full Text PDFGene
January 2025
College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130 China. Electronic address:
White clover (Trifolium repens L.) is a high-quality leguminous forage, but its short rooting habit, poor transpiration tolerance, and drought tolerance, have become a key factor restricting its growth and cultivation. 1R-MYB transcription factors (TFs) are a significant subfamily of TFs in plants, playing a vital role in regulating plant responses to drought stress, however, knowledge about the role of 1R-MYB transcription factors in white clover is still limited.
View Article and Find Full Text PDFFront Plant Sci
January 2025
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Leaf vein, an essential part of leaf architecture, plays significant roles in shaping the proper leaf size. To date, the molecular mechanisms governing leaf development including leaf venation patterning remains poorly understood in birch. Here, we performed the genome-wide identification of homeodomain-like (HD-like) superfamily genes using phylogenetic analysis and revealed the functional role of a potential HD-like gene in leaf growth and development using transgenic technology and transcriptomic sequencing.
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