We describe a microfluidic device designed specifically for the reversible immobilisation of (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4375559 | PMC |
| http://dx.doi.org/10.1016/j.snb.2013.10.002 | DOI Listing |
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