An accurate and sensitive Coomassie Brilliant Blue G-250-based assay for protein determination.

Anal Biochem

Laboratory of Cellular, Nutritional and Toxicological Biochemistry, Institute of Life Sciences, Université Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium.

Published: July 2015

In this protocol we present a rapid and sensitive assay for the accurate determination of protein concentration. The assay is a modification of a previous method, and measures minimum 0.2 μg protein.

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Source
http://dx.doi.org/10.1016/j.ab.2015.03.024DOI Listing

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