AI Article Synopsis
- Mixed-mode chromatography using cysteine-SulfoLink resin effectively purifies ribosomes from cell lysates by removing unwanted proteases and nucleases quickly.
- Binding relies on anion exchange of ribosomal RNA, requiring lysis in a buffer with moderate ionic strength, specifically no more than 20 mS conductivity, and no highly charged additives like heparin.
- The text provides a detailed protocol specifically for purifying ribosomes from Escherichia coli as a practical example.
Article Abstract
Mixed-mode chromatography on cysteine-SulfoLink resin efficiently separates ribosomes from cell lysates and is particularly effective at rapidly removing endogenous proteases and nucleases, resulting in ribosomes of improved purity, integrity, and activity. Binding occurs partly by anion exchange of the RNA of the ribosomes, so that cells must be lysed in a buffer of moderate ionic strength (conductivity no more than 20 mS for chromatography of bacterial ribosomes) without any highly charged additives (e.g., heparin, which is used to inhibit RNases in yeast). A robust protocol for Escherichia coli is given here as an example.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1101/pdb.prot081349 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Clinical and molecular analysis of ESBL, carbapenemase, and colistin-resistant bacteria in UTI patients.
Cell Mol Biol (Noisy-le-grand)
January 2025
Jiangxi Key Laboratory of Oncology (2024SSY06041), Jiangxi Cancer Hospital & Institute, The Second Affiliated Hospital of Nanchang Medical College, Nanchang, Jiangxi, 330029, P.R. China.
Uropathogens, particularly bacteria, can infect any part of the urinary tract and cause bacteriuria. Our study aimed to examine the antibiotic-resistant profile, associated risk factors, and phenotypic and genotypic features of ESBL, carbapenemase, and mcr resistance genes in multidrug-resistant bacteria. Samples were inoculated on culture media, identified using standard biochemical tests, and species confirmation was performed via 16S rRNA gene amplification.
View Article and Find Full Text PDFMicrobial communities associated with the skin, gill, and gut of large yellow croaker (Larimichthys crocea).
BMC Microbiol
January 2025
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou Key Laboratory of Biomedicine and Advanced Dosage Forms, School of Life Sciences, Taizhou University, Taizhou, 318000, Zhejiang Province, China.
The microbiota inhabiting the surface of fish mucosal tissue play important roles in the nutrition, metabolism and immune system of their host. However, most investigations on microbial symbionts have focused on the fish gut, but the microbiota associated with external mucosal tissues (such as the skin and gill) is poorly understood. This study characterised the traits and dynamic of microbial communities associated with the skin, gill and gut of large yellow croaker (Larimichthys crocea) culturing with net enclosures or pens at different sampling times (with seasonal transition).
View Article and Find Full Text PDFDifferences in gut microbial diversity and composition between growth phenotypes of farmed juvenile sandfish, Holothuria scabra.
BMC Microbiol
January 2025
The Marine Science Institute, College of Science, University of the Philippines Diliman, Quezon City, Philippines.
Background: The observed growth variability of different aquaculture species in captivity hinders its large-scale production. For the sandfish Holothuria scabra, a tropical sea cucumber species, there is a scarcity of information on its intestinal microbiota in relation to host growth, which could provide insights into the processes that affect growth and identify microorganisms with probiotic or biochemical potential that could improve current production strategies. To address this gap, this study used 16 S rRNA amplicon sequencing to characterize differences in gut and fecal microbiota among large and small juveniles reared in floating ocean nurseries.
View Article and Find Full Text PDFPredictive Value of a Gastric Microbiota Dysbiosis Test for Stratifying Cancer Risk in Atrophic Gastritis Patients.
Nutrients
December 2024
Oncological Gastroenterology, Centro di Riferimento Oncologico di Aviano (CRO), National Cancer Institute, IRCCS, 33081 Aviano, Italy.
Background/objectives: Gastric cancer (GC) incidence remains high worldwide, and the survival rate is poor. GC develops from atrophic gastritis (AG), associated with () infection, passing through intestinal metaplasia and dysplasia steps. Since eradication does not exclude GC development, further investigations are needed.
View Article and Find Full Text PDFAssociation of Mucin-Degrading Gut Microbiota and Dietary Patterns with Colonic Transit Time in Constipation: A Secondary Analysis of a Randomized Clinical Trial.
Nutrients
December 2024
Department of Bioconvergence, Hoseo University, 165 Sechul-Ri, BaeBang-Yup, Asan 31499, ChungNam-do, Republic of Korea.
Background: The relationship between gut microbiota composition, lifestyles, and colonic transit time (CTT) remains poorly understood. This study investigated associations among gut microbiota profiles, diet, lifestyles, and CTT in individuals with subjective constipation.
Methods: We conducted a secondary analysis of data from our randomized clinical trial, examining gut microbiota composition, CTT, and dietary intake in baseline and final assessments of 94 participants with subjective constipation.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!