AI Article Synopsis
- Transfer RNAs (tRNAs) are essential for translation and have emerging roles in various biological processes.
- The Four-Leaf clover qRT-PCR (FL-PCR) is a newly developed method that accurately quantifies individual mature tRNA species by using a specific ligation technique.
- FL-PCR avoids interference from tRNA modifications, making it a versatile tool for analyzing tRNA levels in different cell types.
Article Abstract
Transfer RNAs (tRNAs) play a central role in translation and also recently appear to have a variety of other functions in biological processes beyond translation. Here we report the development of Four-Leaf clover qRT-PCR (FL-PCR), a convenient PCR-based method, which can specifically quantify individual mature tRNA species. In FL-PCR, T4 RNA ligase 2 specifically ligates a stem-loop adapter to mature tRNAs but not to precursor tRNAs or tRNA fragments. Subsequent TaqMan qRT-PCR amplifies only unmodified regions of the tRNA-adapter ligation products; therefore, FL-PCR quantification is not influenced by tRNA post-transcriptional modifications. FL-PCR has broad applicability for the quantification of various tRNAs in different cell types, and thus provides a much-needed simple method for analyzing tRNA abundance and heterogeneity.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615770 | PMC |
| http://dx.doi.org/10.1080/15476286.2015.1031951 | DOI Listing |
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