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Development of translationally active cell lysates from different filamentous fungi for application in cell-free protein synthesis.
Enzyme Microb Technol
January 2025
Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, Senftenberg 01968, Germany. Electronic address:
There is an enormous potential for cell-free protein synthesis (CFPS) systems based on filamentous fungi in view of their simple, fast and mostly inexpensive cultivation with high biomass space-time yields and in view of their catalytic capacity. In 12 of the 22 different filamentous fungi examined, in vitro translation of at least one of the two reporter proteins GFP and firefly luciferase was detected. The lysates showing translation of a reporter protein usually were able to synthesize a functional cell-free expressed unspecific peroxygenase (UPO) from the basidiomycete Cyclocybe (Agrocybe) aegerita.
View Article and Find Full Text PDFEnhancing farmer awareness: Vertical transmission of Neospora caninum in aborting cattle and the value of diagnostics tools.
Vet Parasitol
January 2025
Institute of Parasitology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, Bern 3012, Switzerland. Electronic address:
The protozoan parasite Neospora caninum is an important cause of abortion in cattle. Infection occurs horizontally by ingestion of oocysts shed by canids or vertically, from an infected dam to the foetus, and may result in abortion, stillbirth, or the birth of subclinically infected offspring. We estimated the occurrence of N.
View Article and Find Full Text PDFNew cinnamic acid sugar esters as potential UVB filters: Synthesis, cytotoxicity, and physicochemical properties.
Carbohydr Res
January 2025
Department of Biomolecular Sciences, University of Urbino "Carlo Bo", Campus Scientifico E. Mattei, via Ca' Le Suore 2, 61029, Urbino, PU, Italy. Electronic address:
Cinnamic Acid Sugar Ester Derivatives (CASEDs) are a class of natural compounds that exhibit several interesting biological activities. However, to date, no examples of their use in sunscreen formulations have been reported. Here, we describe the synthesis of a series of novel cinnamic acid esters of glucose (4a-g), ribose (4h) and lactose (4i) starting from the respective acetals 3.
View Article and Find Full Text PDFAggressiveness and phylogenetic relationship of associated with crown and root rot in pyrethrum plants.
Plant Dis
January 2025
The University of Melbourne, Faculty of Science, School of Agriculture, Food and Ecosystem Sciences, Parkville, Victoria, Australia;
In Australia, pyrethrum (Tanacetum cinerariifolium) cultivation provides a significant portion of the global supply of natural insecticidal pyrethrins. However, crown and root rots, along with stunted plant growth and plant loss during winter, are significant issues affecting certain sites. Several isolates of the Fusarium oxysporum species complex (FOSC) have been identified as causal agents of crown and root rot in pyrethrum, highlighting these as key pathogens contributing to this decline.
View Article and Find Full Text PDFRural and remote health care: the case for spatial justice.
Rural Remote Health
January 2025
School of Health Sciences, Western Sydney University, Campbelltown, NSW 2560, Australia.
Almost universally, people living in rural and remote places die younger, poorer, and sicker than urban-dwelling citizens of the same country. Despite clear need, health services are commonly less available, and more costly and challenging to access, for rural and remote people. Rural geography is commonly cited as a reason for these disparities, that is, rural people are said to live in places too distant, too underpopulated, and too difficult to access.
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