AI Article Synopsis
- The study presents a collection of about 7,434 MiMIC insertions, with 2,854 located in coding introns, leading to the creation of a library with 400 GFP-tagged genes.
- Results show that 72% of the internally tagged proteins are functional, and over 90% of them can be visualized in living tissues.
- The methods developed allow for effective knocking down of tagged mRNAs and proteins, demonstrating significant manipulation capabilities in fruit flies, which may also be applicable to vertebrate studies.
Article Abstract
Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstrate reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379497 | PMC |
| http://dx.doi.org/10.7554/eLife.05338 | DOI Listing |
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