A major challenge in microbial biofilm control is biocide resistance. Phenotypic adaptations and physical protective effects have been historically thought to be the primary mechanisms for glutaraldehyde resistance in bacterial biofilms. Recent studies indicate the presence of genetic mechanisms for glutaraldehyde resistance, but very little is known about the contributory genetic factors. Here, we demonstrate that efflux pumps contribute to glutaraldehyde resistance in Pseudomonas fluorescens and Pseudomonas aeruginosa biofilms. The RNA-seq data show that efflux pumps and phosphonate degradation, lipid biosynthesis, and polyamine biosynthesis metabolic pathways were induced upon glutaraldehyde exposure. Furthermore, chemical inhibition of efflux pumps potentiates glutaraldehyde activity, suggesting that efflux activity contributes to glutaraldehyde resistance. Additionally, induction of known modulators of biofilm formation, including phosphonate degradation, lipid biosynthesis, and polyamine biosynthesis, may contribute to biofilm resistance and resilience. Fundamental understanding of the genetic mechanism of biocide resistance is critical for the optimization of biocide use and development of novel disinfection strategies. Our results reveal genetic components involved in glutaraldehyde resistance and a potential strategy for improved control of biofilms.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4432172 | PMC |
| http://dx.doi.org/10.1128/AAC.05152-14 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Intelligent pH-responsive ratiometric fluorescent nanofiber films for visual and real-time seafood freshness monitoring.
Food Chem
February 2025
Colleage of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China. Electronic address:
In this study, polyvinyl alcohol (PVA)-based ratiometric fluorescence nanofiber films with salicylamide (SA) as the response signal, rhodamine B (RhB) as the internal reference were prepared using electrospinning technique, and reducing their water solubility by glutaraldehyde (G) crosslinked. The RhB and SA were successfully immobilized in the PVA substrate. Moreover, RhB and SA improved the water resistance, water vapor barrier, and mechanical strength of the nanofiber films, among the PVA nanofiber films containing RhB and 400 mg SA (PVA/RhB/4SA-G) showed the best packaging performance.
View Article and Find Full Text PDFEnhancing the Separation Performance of Chitosan Membranes Through the Blending with Deep Eutectic Solvents for the Pervaporation of Polar/Non-Polar Organic Mixtures.
Membranes (Basel)
November 2024
Institute on Membrane Technology (CNR-ITM), Via P. Bucci 17/c, 87036 Rende, CS, Italy.
Article Synopsis
- - The study investigates chitosan-based membranes mixed with eco-friendly deep eutectic solvents (DESs) to improve the separation of methanol and methyl-butyl ether via pervaporation.
- - Results showed that adding DESs boosted the membranes' selective permeability for methanol by up to three times compared to regular chitosan membranes, with performance influenced by feed temperature.
- - Crosslinking the membranes with glutaraldehyde enhanced their selectivity, highlighting the potential of DESs to improve biopolymer membranes for efficient and environmentally friendly separations.
Hybrid magnetic nanocomposites of arginine deiminase with improved stability and recyclability for biomedical applications.
Prep Biochem Biotechnol
November 2024
Medical Microbiology and Bioprocess Technology Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, India.
Nanocarrier-based immobilization has created new avenues for enhancing the biophysical properties of enzymes. Nanomatrices such as magnetite nanoparticles (MNPs), chitin, and chitosan with large surface areas and tunable morphology have been developed to circumvent the bottlenecks of free enzymes. The present study used MNPs to immobilize the enzyme arginine deiminase (ADI) for improved morphological control, recovery, operational stability, and easy recyclability.
View Article and Find Full Text PDFPreparation and properties of room temperature foaming lignin-based non-isocyanate polyurethane foams.
Int J Biol Macromol
December 2024
Yunnan Key Laboratory of Wood Adhesives and Glued Products, College of Material science and Chemistry Engineering, Southwest Forestry University, 650224 Kunming, China. Electronic address:
Article Synopsis
- Biomass-based non-isocyanate polyurethane (NIPU) is gaining attention in the polyurethane industry for its sustainable materials and lack of toxic isocyanates.
- This study focuses on lignin, a renewable natural polymer, to create lignin-based NIPU (L-NIPU) foams using maleic acid as an initiator and glutaraldehyde as a cross-linker, producing lightweight and strong materials.
- The optimal composition of 18% maleic acid and 25% glutaraldehyde yields foams with significant compressive strength (up to 0.5 MPa) and low thermal conductivity (0.03559 W/m·K), making them suitable for insulation in construction
Flexible Highly Thermally Conductive PCM Film Prepared by Centrifugal Electrospinning for Wearable Thermal Management.
Materials (Basel)
October 2024
Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources, Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences (Beijing), Beijing 100083, China.
Personal thermal management materials integrated with phase-change materials have significant potential to satisfy human thermal comfort needs and save energy through the efficient storage and utilization of thermal energy. However, conventional organic phase-change materials in a solid state suffer from rigidity, low thermal conductivity, and leakage, making their application challenging. In this work, polyethylene glycol (PEG) was chosen as the phase-change material to provide the energy storage density, polyethylene oxide (PEO) was chosen to provide the backbone structure of the three-dimensional polymer network and cross-linked with the PEG to provide flexibility, and carbon nanotubes (CNTs) were used to improve the mechanical and thermal conductivity of the material.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!