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Expression of 1N3R-Tau isoform inhibits cell proliferation by inducing S phase arrest in N2a cells. | LitMetric

Expression of 1N3R-Tau isoform inhibits cell proliferation by inducing S phase arrest in N2a cells.

PLoS One

Department of Pathophysiology, Key Laboratory of Education Ministry of China for Neurological Disorders, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.

Published: April 2016

AI Article Synopsis

  • Tau is a protein associated with microtubules, linked to neurodegenerative diseases, and exists in six different isoforms produced through alternative splicing.
  • The study focused on understanding how these tau isoforms affect cell proliferation by using N2a cells and monitoring the expressions of green fluorescent protein (eGFP).
  • Results revealed that the 1N3R-tau isoform significantly decreased cell proliferation and induced S phase arrest, indicating its unique role in regulating the cell cycle compared to other tau isoforms.

Article Abstract

Tau is a microtubule-associated protein implicated in neurodegenerative tauopathies. Six tau isoforms are generated from a single gene through alternative splicing of exons 2, 3 and 10 in human brain. Differential expression of tau isoforms has been detected in different brain areas, during neurodevelopment and in neurodegenerative disorders. However, the biological significance of different tau isoforms is not clear. Here, we investigated the individual effect of six different isoforms of tau on cell proliferation and the possible mechanisms by transient expression of eGFP-labeled tau isoform plasmid in N2a cells. Our study showed the transfection efficiency was comparable between different isoforms of tau by examining GFP expression. Compared with other isoforms, we found expression of 1N3R-tau significantly inhibited cell proliferation by Cell Counting Kit-8 assay and BrdU incorporation. Flow cytometry analysis further showed expression of 1N3R-tau induced S phase arrest. Compared with the longest isoform of tau, expression of 1N3R-tau induced cyclin E translocation from the nuclei to cytoplasm, while it did not change the level of cell cycle checkpoint proteins. These data indicate that 1N3R-tau inhibits cell proliferation through inducing S phase arrest.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4378987PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119865PLOS

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