AI Article Synopsis
- Polymerized high internal phase emulsion (polyHIPE) foams can be tailored for studying cell-substrate interactions, allowing researchers to create various pore structures and compare 2D and 3D environments with the same surface chemistry.
- Using amphiphilic block copolymers, the study controls how ligands are arranged on the foam surface, influencing cell adhesion of human mesenchymal progenitor (hES-MP) cells.
- The findings reveal that surface topology and chemistry affect cell adhesion and osteogenic differentiation, suggesting that the design of 3D scaffolds can regulate stem cell behavior independent of growth factors.
Article Abstract
Polymerized high internal phase emulsion (polyHIPE) foams are extremely versatile materials for investigating cell-substrate interactions in vitro. Foam morphologies can be controlled by polymerization conditions to result in either open or closed pore structures with different levels of connectivity, consequently enabling the comparison between 2D and 3D matrices using the same substrate with identical surface chemistry conditions. Additionally, here we achieve the control of pore surface topology (i.e. how different ligands are clustered together) using amphiphilic block copolymers as emulsion stabilizers. We demonstrate that adhesion of human mesenchymal progenitor (hES-MP) cells cultured on polyHIPE foams is dependent on foam surface topology and chemistry but is independent of porosity and interconnectivity. We also demonstrate that the interconnectivity, architecture and surface topology of the foams has an effect on the osteogenic differentiation potential of hES-MP cells. Together these data demonstrate that the adhesive heterogeneity of a 3D scaffold could regulate not only mesenchymal stem cell attachment but also cell behavior in the absence of soluble growth factors.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4379418 | PMC |
| http://dx.doi.org/10.1016/j.biomaterials.2015.01.034 | DOI Listing |
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