This communication describes the general characteristics of the venom from the Brazilian scorpion Tityus fasciolatus, which is an endemic species found in the central Brazil (States of Goiás and Minas Gerais), being responsible for sting accidents in this area. The soluble venom obtained from this scorpion is toxic to mice being the LD50 is 2.984 mg/kg (subcutaneally). SDS-PAGE of the soluble venom resulted in 10 fractions ranged in size from 6 to 10-80 kDa. Sheep were employed for anti-T. fasciolatus venom serum production. Western blotting analysis showed that most of these venom proteins are immunogenic. T. fasciolatus anti-venom revealed consistent cross-reactivity with venom antigens from Tityus serrulatus. Using known primers for T. serrulatus toxins, we have identified three toxins sequences from T. fasciolatus venom. Linear epitopes of these toxins were localized and fifty-five overlapping pentadecapeptides covering complete amino acid sequence of the three toxins were synthesized in cellulose membrane (spot-synthesis technique). The epitopes were located on the 3D structures and some important residues for structure/function were identified.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.toxicon.2015.03.018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of Synthetic Tf2 as a Na1.3 Selective Pharmacological Probe.
Biomedicines
June 2020
Institute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, Australia.
Na1.3 is a subtype of the voltage-gated sodium channel family. It has been implicated in the pathogenesis of neuropathic pain, although the contribution of this channel to neuronal excitability is not well understood.
View Article and Find Full Text PDFSubtype Specificity of β-Toxin Tf1a from in Voltage Gated Sodium Channels.
Toxins (Basel)
August 2018
Laboratório de Neurofarmacologia, Departamento de Ciências Biológicas, Universidade de Brasília, Brasília 70910-900, Distrito Federal, Brazil.
Scorpion venoms are a complex mixture of components. Among them the most important are peptides, which presents the capacity to interact and modulate several ion channel subtypes, including voltage-gated sodium channels (Na). Screening the activity of scorpion toxins on different subtypes of Na reveals the scope of modulatory activity and, in most cases, low channel selectivity.
View Article and Find Full Text PDFEffects of Brazilian scorpion venoms on the central nervous system.
J Venom Anim Toxins Incl Trop Dis
January 2018
1Laboratory of Pharmacology, Butantan Institute, Av. Dr. Vital Brasil, 1500, São Paulo, SP 05503-900 Brazil.
In Brazil, the scorpion species responsible for most severe incidents belong to the genus and, among this group, , , and are the most dangerous ones. Other species such as , , , , are also found in the country, but the incidence and severity of accidents caused by them are lower. The main effects caused by scorpion venoms - such as myocardial damage, cardiac arrhythmias, pulmonary edema and shock - are mainly due to the release of mediators from the autonomic nervous system.
View Article and Find Full Text PDFThe Scorpion Toxin Tf2 from Tityus fasciolatus Promotes Nav1.3 Opening.
PLoS One
March 2016
Departamento de Ciências Fisiológicas, Laboratório de Toxinologia, Universidade de Brasília, Brasília, DF, Brazil.
We identified Tf2, the first β-scorpion toxin from the venom of the Brazilian scorpion Tityus fasciolatus. Tf2 is identical to Tb2-II found in Tityus bahiensis. We found that Tf2 selectively activates human (h)Nav1.
View Article and Find Full Text PDFGeneral characterization of Tityus fasciolatus scorpion venom. Molecular identification of toxins and localization of linear B-cell epitopes.
Toxicon
June 2015
Departamento de Bioquímica-Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CP: 486, CEP: 31270-901 Belo Horizonte, MG, Brazil. Electronic address:
This communication describes the general characteristics of the venom from the Brazilian scorpion Tityus fasciolatus, which is an endemic species found in the central Brazil (States of Goiás and Minas Gerais), being responsible for sting accidents in this area. The soluble venom obtained from this scorpion is toxic to mice being the LD50 is 2.984 mg/kg (subcutaneally).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!