Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/ncb3153 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Organization of the chromosomal passenger complex clusters at inner centromeres in mitosis.
Curr Opin Cell Biol
February 2025
Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research (JFCR), Tokyo, Japan; Department of JFCR Cancer Biology, Institute of Science Tokyo, Tokyo, Japan. Electronic address:
Stable transmission of the genome during cell division is crucial for all life forms and is universally achieved by Aurora B-mediated error correction of the kinetochore-microtubule attachments. Aurora B is the enzymatic subunit of the tetrameric protein complex called the chromosomal passenger complex (CPC), and its centromeric enrichment is required for Aurora B to ensure accurate chromosome segregation. How cells enrich the CPC at centromeres is therefore an outstanding question to be elucidated.
View Article and Find Full Text PDFThe dependence of shugoshin on Bub1-kinase activity is dispensable for the maintenance of spindle assembly checkpoint response in Cryptococcus neoformans.
PLoS Genet
January 2025
Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India.
During chromosome segregation, the spindle assembly checkpoint (SAC) detects errors in kinetochore-microtubule attachments. Timely activation and maintenance of the SAC until defects are corrected is essential for genome stability. Here, we show that shugoshin (Sgo1), a conserved tension-sensing protein, ensures the maintenance of SAC signals in response to unattached kinetochores during mitosis in a basidiomycete budding yeast Cryptococcus neoformans.
View Article and Find Full Text PDFCENP-E haploinsufficiency causes chromosome misalignment and spindle assembly checkpoint activation in the spermatogonia.
Andrology
December 2024
Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian, China.
Background: The establishment of kinetochore-microtubule attachment is essential for error-free chromosome alignment and segregation during cell division. Defects in chromosome alignment result in chromosome instability, birth defects, and infertility. Kinesin-7 CENP-E mediates kinetochore-microtubule capture, chromosome alignment, and spindle assembly checkpoint in somatic cells, however, mechanisms of CENP-E in germ cells remain poorly understood.
View Article and Find Full Text PDFSuper-Resolution Imaging of Mitotic Spindle Microtubules Using STED Microscopy.
Methods Mol Biol
December 2024
Division of Molecular Biology, Ruđer Bošković Institute, Zagreb, Croatia.
Article Synopsis
- STED microscopy is a cutting-edge imaging technique that is now being utilized in the study of mitosis, specifically for investigating microtubules, kinetochore-microtubule dynamics, and chromosome segregation issues.
- The method combines immunofluorescence with STED microscopy to analyze human mitotic spindles, allowing for detailed observation of microtubule attachments during cell division.
- Live-cell STED microscopy allows for short-term imaging of dynamic processes in spindle regions, and various image analysis techniques are proposed for quantitatively assessing microtubule bundles.
CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation.
Life Sci Alliance
January 2025
Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan
Establishing the correct kinetochore-microtubule attachment is crucial for faithful chromosome segregation. The kinetochore has various regulatory mechanisms for establishing correct bipolar attachment. However, how the regulations are coupled is not fully understood.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!